New Insights in Trichoderma harzianum Antagonism of Fungal Plant Pathogens by Secreted Protein Analysis

• Published in: Current microbiology Vol. 61; no. 4; pp. 298 - 305
• Main Authors: Monteiro, Valdirene Neves, do Nascimento Silva, Roberto, Steindorff, Andrei Stecca, Costa, Fabio Teles, Noronha, Eliane Ferreira, Ricart, Carlos André Ornelas, de Sousa, Marcelo Valle, Vainstein, Marilene Henning, Ulhoa, Cirano José
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01-10-2010; Springer-Verlag; Springer Nature B.V
• Subjects: Acid Phosphatase - metabolism; alpha-Mannosidase - metabolism; Antibiosis; Ascomycota - physiology; beta-Glucosidase - metabolism; Biomedical and Life Sciences; Biotechnology; Cell Wall - genetics; Cell Wall - metabolism; Chitinases - metabolism; Electrophoresis, Gel, Two-Dimensional; Fungal Proteins - genetics; Fungal Proteins - isolation & purification; Fungal Proteins - metabolism; Fungi; Fusarium - physiology; Growth conditions; Life Sciences; Mass Spectrometry; Microbiology; Microscopy, Electron, Scanning; Pathogens; Pest Control, Biological; Plant pathology; Proteins; Proteomics; Rhizoctonia - physiology; Trichoderma - growth & development; Trichoderma - metabolism; Trichoderma - physiology; Fusarium; Trichoderma; Chitinases; Rhizoctonia Solani; Trichoderma Harzianum
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-010-9611-8

Trichoderma harzianum ALL42 were capable of overgrowing and degrading Rhizoctonia solani and Macrophomina phaseolina mycelia, coiling around the hyphae with formation of apressoria and hook-like structures. Hyphae of T. harzianum ALL42 did not show any coiling around Fusarium sp. hyphae suggesting that mycoparasitism may be different among the plant pathogens. In this study, a secretome analysis was used to identify some extracellular proteins secreted by T. harzianum ALL42 after growth on cell wall of M. phaseolina, Fusarium sp., and R. solani. The secreted proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. A total of 60 T. harzianum ALL42 secreted proteins excised from the gel were analyzed from the three growth conditions. While seven cell wall-induced proteins were identified, more than 53 proteins spots remain unidentified, indicating that these proteins are either novel proteins or proteins that have not yet been sequenced. Endochitinase, β-glucosidase, α-mannosidase, acid phosphatase, α-1,3-glucanase, and proteases were identified in the gel and also detected in the supernatant of culture.