Targeted Interneuron Depletion in the Dorsal Striatum Produces Autism-like Behavioral Abnormalities in Male but Not Female Mice

• Published in: Biological psychiatry (1969) Vol. 82; no. 3; pp. 194 - 203
• Main Authors: Rapanelli, Maximiliano, Frick, Luciana Romina, Xu, Meiyu, Groman, Stephanie Mary, Jindachomthong, Kantiya, Tamamaki, Nobuaki, Tanahira, Chiyoko, Taylor, Jane Rebecca, Pittenger, Christopher
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-08-2017
• Subjects: Animals; Anxiety; Anxiety - pathology; Anxiety - physiopathology; Autism spectrum disorder; Autistic Disorder - pathology; Autistic Disorder - physiopathology; Conditioning, Operant - physiology; Corpus Striatum - pathology; Corpus Striatum - physiopathology; Disease Models, Animal; Exploratory Behavior - physiology; Female; Immunohistochemistry; Interneuron; Interneurons - pathology; Interneurons - physiology; Male; Mice, Transgenic; Motor Activity - physiology; Prepulse Inhibition - physiology; Psychiatry; Ribosomal Protein S6 Kinases, 90-kDa - metabolism; Sex Characteristics; Social Behavior; Social preference; Stereotyped Behavior - physiology; Stereotypy; Striatum; Synaptic Transmission - physiology; Social preference; Striatum; Anxiety; Stereotypy; Autism spectrum disorder; Interneuron
• ISSN: 0006-3223; 1873-2402
• DOI: 10.1016/j.biopsych.2017.01.020

Abstract Background Interneuronal pathology is implicated in many neuropsychiatric disorders, including autism spectrum disorder (ASD) and Tourette syndrome (TS). Interneurons of the striatum, including the parvalbumin-expressing fast-spiking interneurons (FSIs) and the large cholinergic interneurons (CINs), are affected in patients with TS and in preclinical models of both ASD and TS. Methods To test the causal importance of these neuronal abnormalities, we recapitulated them in vivo in developmentally normal mice using a combination transgenic–viral strategy for targeted toxin-mediated ablation. Results We found that conjoint ~50% depletion of FSIs and CINs in the dorsal striatum of male mice produces spontaneous stereotypy and marked deficits in social interaction. Strikingly, these behavioral effects are not seen in female mice; because ASD and TS have a marked male predominance, this observation reinforces the potential relevance of the finding to human disease. Neither of these effects is seen when only one or the other interneuronal population is depleted; ablation of both is required. Depletion of FSIs, but not of CINs, also produces anxiety-like behavior, as has been described previously. Behavioral pathology in male mice after conjoint FSI and CIN depletion is accompanied by increases in activity-dependent signaling in the dorsal striatum; these alterations were not observed after disruption of only one interneuron type or in doubly depleted female mice. Conclusions These data indicate that disruption of CIN and FSI interneurons in the dorsal striatum is sufficient to produce network and behavioral changes of potential relevance to ASD, in a sexually dimorphic manner.