Vaccination with NS1-truncated H3N2 swine influenza virus primes T cells and confers cross-protection against an H1N1 heterosubtypic challenge in pigs

• Published in: Vaccine Vol. 30; no. 2; pp. 280 - 288
• Main Authors: Kappes, Matthew A, Sandbulte, Matthew R, Platt, Ratree, Wang, Chong, Lager, Kelly M, Henningson, Jamie N, Lorusso, Alessio, Vincent, Amy L, Loving, Crystal L, Roth, James A, Kehrli, Marcus E
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 05-01-2012; Elsevier Limited
• Subjects: Allergy and Immunology; Animals; Animals, Newborn; Cross Protection; Disease Models, Animal; Eggs; Heterosubtypic cross protection; Hogs; Influenza A Virus, H1N1 Subtype - immunology; Influenza A Virus, H3N2 Subtype - genetics; Influenza A Virus, H3N2 Subtype - immunology; Influenza Vaccines - administration & dosage; Influenza Vaccines - genetics; Influenza Vaccines - immunology; Lymphocytes; Modified live vaccine; Mutation; Orthomyxoviridae Infections - immunology; Orthomyxoviridae Infections - prevention & control; Population; Proteins; Swine; Swine flu; Swine influenza virus; T cell receptors; T-cell immunity; T-Lymphocytes - immunology; Vaccines; Vaccines, Attenuated - administration & dosage; Vaccines, Attenuated - genetics; Vaccines, Attenuated - immunology; Viral Nonstructural Proteins - genetics; Viruses; Swine influenza virus; T-cell immunity; Heterosubtypic cross protection; Modified live vaccine
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2011.10.098

Highlights ► Attenuated H3N2 influenza vaccine with truncated NS1 was administered to pigs. ► Vaccine induced broadly reactive mucosal antibodies. ► Vaccine primed T cells which were elevated in peripheral blood after infection. ► Vaccinated animals had reduced shedding of heterosubtypic H1N1 challenge virus.