Monoclonal Antibodies Specific for p24, the Major Core Protein of Human T-Cell Leukemia Virus Type III

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 82; no. 15; pp. 5199 - 5202
• Main Authors: F. Di Marzo Veronese, Sarngadharan, M. G., Rahman, R., Markham, P. D., Popovic, M., Bodner, A. J., Gallo, Robert C.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01-08-1985; National Acad Sciences
• Subjects: Acquired Immunodeficiency Syndrome - diagnosis; AIDS; AIDS/HIV; Antibodies; Antibodies, immunoglobulins; Antibodies, Monoclonal - immunology; Antibodies, Viral - immunology; Antibody Specificity; Biological and medical sciences; Deltaretrovirus - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; HIV; Human T lymphotropic virus 1; Humans; Hybridomas; Lymphocytes; Molecular immunology; Molecular Weight; Monoclonal antibodies; Reactivity; T lymphocytes; Viral Core Proteins; Viral Proteins - immunology; Viruses; Human; Cell culture; Oncovirinae; Rodentia; Immunoreagent; Retroviridae; Hybridoma; AIDS; Monoclonal antibody; Virus; Proteins; Vertebrata; Mammalia; Mouse; Animal; Type C oncovirus; Production
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.82.15.5199

Four mouse hybridomas secreting monoclonal antibodies specific for p24, the major core antigen of the human T-cell leukemia virus type III (HTLV-III), have been developed, and their specificities have been partially characterized. These antibodies specifically recognized p24 of HTLV-III in extracts of HTLV-III and in HTLV-III-producing cells. No epitopes cross-reactive with HTLV-I and -II were detected with these antibodies. These hybridomas will be extremely valuable reagents in identifying expression of HTLV-III in infected cultures and in cells or tissues from patients with suspected immunodeficiency syndrome.