Trans-acting small interfering RNA4: key to nutraceutical synthesis in grape development?

• Published in: Trends in plant science Vol. 18; no. 11; pp. 601 - 610
• Main Author: Rock, Christopher D
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-11-2013
• Subjects: biosynthesis; breeding; Dietary Supplements; DNA Shuffling; evolution; foods; Fruit - chemistry; Fruit - genetics; Fruit - growth & development; Fruit - physiology; fruiting; Gene Expression Regulation, Plant; genes; Genetic Engineering; grapes; microRNA; Mycorrhizae - physiology; pathogens; polyphenols; Polyphenols - biosynthesis; RNA, Plant - genetics; RNA, Small Interfering - genetics; Signal Transduction; small interfering RNA; Symbiosis; transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Vitaceae; Vitis - chemistry; Vitis - genetics; Vitis - growth & development; Vitis - physiology; Vitis vinifera
• ISSN: 1360-1385; 1878-4372
• DOI: 10.1016/j.tplants.2013.07.006

The facility and versatility of microRNAs (miRNAs) to evolve and change likely underlies how they have become dominant constituents of eukaryotic genomes. In this opinion article I propose that trans-acting small interfering RNA gene 4 (TAS4) evolution may be important for biosynthesis of polyphenolics, arbuscular symbiosis, and bacterial pathogen etiologies. Expression-based and phylogenetic evidence shows that TAS4 targets two novel grape (Vitis vinifera L.) MYB transcription factors (VvMYBA6, VvMYBA7) that spawn phased small interfering RNAs (siRNAs) which probably function in nutraceutical bioflavonoid biosynthesis and fruit development. Characterization of the molecular mechanisms of TAS4 control of plant development and integration into biotic and abiotic stress- and nutrient-signaling regulatory networks has applicability to molecular breeding and the development of strategies for engineering healthier foods.