New insights into the structure and mode of action of Mo-CBP3, an antifungal chitin-binding protein of Moringa oleifera seeds

• Published in: PloS one Vol. 9; no. 10; p. e111427
• Main Authors: Batista, Adelina B, Oliveira, José T A, Gifoni, Juliana M, Pereira, Mirella L, Almeida, Marina G G, Gomes, Valdirene M, Da Cunha, Maura, Ribeiro, Suzanna F F, Dias, Germana B, Beltramini, Leila M, Lopes, José Luiz S, Grangeiro, Thalles B, Vasconcelos, Ilka M
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27-10-2014; Public Library of Science (PLoS)
• Subjects: Antifungal activity; Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Antimicrobial agents; Apoptosis; Biochemistry; Biology and Life Sciences; Candida albicans; Cell surface; Chitin; Chitin - metabolism; Circular dichroism; Colletotrichum - drug effects; Cytoplasm; Cytotoxicity; Dichroism; Drug development; Electrostatic properties; Fungi; Fungicides; Fusarium - drug effects; Germination; Helices; Mode of action; Molecular biology; Moringa oleifera; Moringa oleifera - chemistry; Mycelia; Oxygen; Peptides; Phytopathogenic fungi; Plant Proteins - chemistry; Plant Proteins - pharmacology; Protein Binding; Protein Stability; Proteins; Reactive oxygen species; Salts; Seeds; Seeds - chemistry; Spectroscopy; Spore germination; Spores, Fungal - drug effects; Toxicity; Transgenic plants; Brazil; Rio de Janeiro Brazil
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0111427

Mo-CBP3 is a chitin-binding protein purified from Moringa oleifera Lam. seeds that displays inhibitory activity against phytopathogenic fungi. This study investigated the structural properties and the antifungal mode of action of this protein. To this end, circular dichroism spectroscopy, antifungal assays, measurements of the production of reactive oxygen species and microscopic analyses were utilized. Mo-CBP3 is composed of 30.3% α-helices, 16.3% β-sheets, 22.3% turns and 30.4% unordered forms. The Mo-CBP3 structure is highly stable and retains its antifungal activity regardless of temperature and pH. Fusarium solani was used as a model organism for studying the mechanisms by which this protein acts as an antifungal agent. Mo-CBP3 significantly inhibited spore germination and mycelial growth at 0.05 mg.mL-1. Mo-CBP3 has both fungistatic and fungicidal effects, depending on the concentration used. Binding of Mo-CBP3 to the fungal cell surface is achieved, at least in part, via electrostatic interactions, as salt was able to reduce its inhibitory effect. Mo-CBP3 induced the production of ROS and caused disorganization of both the cytoplasm and the plasma membrane in F. solani cells. Based on its high stability and specific toxicity, with broad-spectrum efficacy against important phytopathogenic fungi at low inhibitory concentrations but not to human cells, Mo-CBP3 has great potential for the development of new antifungal drugs or transgenic crops with enhanced resistance to phytopathogens.