Vaccinium macrocarpon Aiton Extract Ameliorates Inflammation and Hyperalgesia through Oxidative Stress Inhibition in Experimental Acute Pancreatitis

• Published in: Evidence-based complementary and alternative medicine Vol. 2018; no. 2018; pp. 1 - 13
• Main Authors: Camargo, Enilton A., Silva, Ana Mara de Oliveira e, Hassimotto, Neuza Mariko Aymoto, Santos, José Thiago do Carmo, Souza, Marília Trindade de Santana, Oliveira, Alan Santos, Santana, Danielle G., Grespan, Renata
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2018; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Analysis; Anthocyanin; Anthocyanins; Arginine; Catalase; Catechin; Disease; Edema; Epicatechin; Flavonoids; Gastroenterology; Glutathione peroxidase; Hyperalgesia; Inflammation; Interleukin 6; Laboratory animals; Metastases; Mortality; Oxidative stress; Pain perception; Pancreas; Pancreatitis; Rutin; Solvents; Sulfhydryl groups; Superoxide dismutase; Tumor necrosis factor-α; Urogenital system; Vaccinium macrocarpon; Brazil; Illinois
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2018/9646937

We evaluated the effect of the hydroethanolic extract of fruits of Vaccinium macrocarpon (HEVm) in a model of acute pancreatitis (AP) in mice. AP was induced by two injections of L-arginine and animals were treated with HEVm (50, 100, and 200 mg/kg, p.o.) or vehicle (saline) every 24 h, starting 1 h after the induction of AP. Phytochemical analysis of the extract and measurement of inflammatory and oxidative stress parameters, as well as abdominal hyperalgesia, were performed. Catechin, epicatechin, rutin, and anthocyanins were identified in HEVm. Treatment with HEVm decreased L-arginine-induced abdominal hyperalgesia (from 48 to 72 h). Also, treatment with HEVm decreased L-arginine-induced pancreatic edema, pancreatic and pulmonary neutrophil infiltration, and levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6, after 72 h of induction. L-arginine-induced hyperamylasemia and hyperlipasemia were also reduced by the treatment with HEVm in comparison to vehicle-treated group. Moreover, lipoperoxidation, carbonyl radicals, nonprotein sulfhydryl groups, and activity of catalase and superoxide dismutase, but not glutathione peroxidase, were restored by the treatment with HEVm. These results show that treatment with HEVm decreased hyperalgesia and pancreatic/extrapancreatic inflammation and oxidative damage in L-arginine-induced AP, making this extract attractive for future approaches designed to treat this condition.