Association of lipoprotein lipase gene polymorphisms with obesity and type 2 diabetes in an Asian Indian population

• Published in: International Journal of Obesity Vol. 31; no. 6; pp. 913 - 918
• Main Authors: Radha, V, Vimaleswaran, K.S, Ashok Ayyappa, K, Mohan, V
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 01-06-2007; Nature Publishing Group
• Subjects: adults; Biological and medical sciences; Body Mass Index; Case-Control Studies; Cholesterol; Control; Diabetes; Diabetes Mellitus, Type 2 - epidemiology; Diabetes Mellitus, Type 2 - genetics; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Enzymes; Epidemiology; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Gene Frequency - genetics; Genetic aspects; genetic polymorphism; Genetic polymorphisms; genetic variation; Genotype; Genotype & phenotype; Glucose; Humans; Hydrolysis; India - epidemiology; Insulin resistance; linkage disequilibrium; Linkage Disequilibrium - genetics; lipid metabolism; lipoprotein lipase; Lipoprotein Lipase - genetics; Lipoproteins; Male; Medical sciences; Metabolic diseases; Metabolism; Middle Aged; molecular sequence data; noninsulin-dependent diabetes mellitus; Obesity; Obesity - epidemiology; Obesity - genetics; Physiological aspects; Polymerase chain reaction; Polymorphism, Genetic - genetics; Population studies; promoter regions; Promoter Regions, Genetic - genetics; restriction fragment length polymorphism; Risk factors; single nucleotide polymorphism; triacylglycerols; Triglycerides; Type 2 diabetes; Urban Health; Vertebrates: anatomy and physiology, studies on body, several organs or systems; very low density lipoprotein; waist circumference; Waist-Hip Ratio; India; Asia; Germany; Endocrinopathy; Type 2 diabetes; Human; Obesity; Asiatic; Genetic variability; Nutrition; Enzyme; Nutrition disorder; Genotype; Metabolic diseases; LPL; Esterases; Lipoprotein lipase; Asian Indians; Carboxylic ester hydrolases; Association; Hydrolases; Genetics; Indian; Population; Public health; Nutritional status; Polymorphism
• ISSN: 0307-0565; 1476-5497
• DOI: 10.1038/sj.ijo.0803547

Aims: Lipoprotein lipase (LPL), a pivotal enzyme in lipoprotein metabolism, catalyzes the hydrolysis of triglycerides of very low-density lipoproteins and chylomicrons. Assuming that the variants in the promoter of the LPL gene may be associated with changes in lipid metabolism leading to obesity and type 2 diabetes, we examined the role of promoter variants (-T93G and -G53C) in the LPL gene in an urban South Indian population. Methods: The study subjects (619 type 2 diabetic and 731 normal glucose-tolerant (NGT) subjects) were chosen from the Chennai Urban Rural Epidemiology Study, an ongoing population-based study in southern India. The polymorphisms were genotyped using polymerase chain reaction-restriction-fragment length polymorphism (PCR-RFLP). Linkage disequilibrium (LD) was estimated from the estimates of haplotypic frequencies. Results: The two polymorphisms studied were not in LD. The -T93G was not associated with type 2 diabetes but was associated with obesity. 11.5% of the obese subjects (62/541) had the XG(TG+GG) genotype compared with 6.4% of the nonobese subjects (52/809; P=0.001). The odds ratio for obesity for the XG genotype was 1.766 (95% CI: 1.19-2.63, P=0.005). Subjects with XG genotype also had higher body mass index and waist circumference compared with those with TT genotype. With respect to G53C, subjects with the XC(GC+CC) genotype had 0.527 and 0.531 times lower risk for developing type 2 diabetes and obesity, respectively. Conclusions: Among Asian Indians, the -T93G SNP of the LPL gene is associated with obesity but not type 2 diabetes, whereas the -G53C SNP appears to be protective against both obesity and type 2 diabetes.