Novel mutations in ndh in isoniazid-resistant Mycobacterium tuberculosis isolates

• Published in: Antimicrobial agents and chemotherapy Vol. 45; no. 7; pp. 2157 - 2159
• Main Authors: LEE, Ann S. G, TEO, Audrey S. M, WONG, Sin-Yew
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-07-2001
• Series: Note
• Subjects: Amino Acid Sequence; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antitubercular Agents - pharmacology; Bacteriology; Biological and medical sciences; Drug Resistance, Microbial - genetics; Fundamental and applied biological sciences. Psychology; Genetics; Genotype; Humans; isoniazid; Isoniazid - pharmacology; Mechanisms of Resistance; Medical sciences; Microbial Sensitivity Tests; Microbiology; Molecular Sequence Data; Mutation; Mycobacterium tuberculosis; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Mycobacterium tuberculosis - metabolism; NADH Dehydrogenase - genetics; ndh gene; Pharmacology. Drug treatments; Polymerase Chain Reaction; Sequence Homology, Amino Acid; Human; Nucleotide sequence; Enzyme; NADH dehydrogenase; In vitro; Resistance; Gene; Mycobacterium tuberculosis; Mycobacteriales; Mycobacteriaceae; Bacteria; Genetics; Actinomycetes; Antituberculous agent; Oxidoreductases; Mutation; Antibacterial agent; Mechanism of action; Clinical isolate; Isoniazid
• ISSN: 0066-4804; 1098-6596
• DOI: 10.1128/AAC.45.7.2157-2159.2001

Novel mutations in NADH dehydrogenase (ndh) were detected in 8 of 84 (9.5%) isoniazid (INH)-resistant isolates (T110A [n = 1], R268H [n = 7]), but not in 22 INH-susceptible isolates of Mycobacterium tuberculosis. Significantly, all eight isolates with mutations at ndh did not have mutations at katG, kasA, or the promoter regions of inhA or ahpC, except for one isolate. Mutations in ndh appear to be an additional molecular mechanism for isoniazid resistance in M. tuberculosis.