Dark fermentation of complex waste biomass for biohydrogen production by pretreated thermophilic anaerobic digestate

• Published in: Journal of environmental management Vol. 152; pp. 43 - 48
• Main Authors: Ghimire, Anish, Frunzo, Luigi, Pontoni, Ludovico, d'Antonio, Giuseppe, Lens, Piet N.L., Esposito, Giovanni, Pirozzi, Francesco
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-04-2015; Academic Press Ltd
• Subjects: Agriculture; Alkanesulfonic Acids - metabolism; Anaerobiosis; Besa; Biofuels; Biohydrogen; Biomass; Dark fermentation; Environmental Restoration and Remediation - methods; Fermentation; Hydrogen; Hydrogen - metabolism; Industrial Waste - analysis; Inhibitors; Italy; Metabolites; Olea; Olives; Pathways; Pumpkins; Sludge; Solanum tuberosum; Substrates; Waste biomass; Waste valorization; Wastes; Italy; Italy, Campania; MED, Italy; Waste biomass; Waste valorization; Biohydrogen; Biofuels; Dark fermentation
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2014.12.049

The Biohydrogen Potential (BHP) of six different types of waste biomass typical for the Campania Region (Italy) was investigated. Anaerobic sludge pre-treated with the specific methanogenic inhibitor sodium 2-bromoethanesulfonic acid (BESA) was used as seed inoculum. The BESA pre-treatment yielded the highest BHP in BHP tests carried out with pre-treated anaerobic sludge using potato and pumpkin waste as the substrates, in comparison with aeration or heat shock pre-treatment. The BHP tests carried out with different complex waste biomass showed average BHP values in a decreasing order from potato and pumpkin wastes (171.1 ± 7.3 ml H2/g VS) to buffalo manure (135.6 ± 4.1 ml H2/g VS), dried blood (slaughter house waste, 87.6 ± 4.1 ml H2/g VS), fennel waste (58.1 ± 29.8 ml H2/g VS), olive pomace (54.9 ± 5.4 ml H2/g VS) and olive mill wastewater (46.0 ± 15.6 ml H2/g VS). The digestate was analyzed for major soluble metabolites to elucidate the different biochemical pathways in the BHP tests. These showed the H2 was produced via mixed type fermentation pathways.
•The BHP of agro-industrial waste biomass from Campania Region (Italy) is evaluated.•Agro-industrial wastes are promising substrates for DF biohydrogenation.•BESA effectively prepares the hydrogen producing seed inoculum.•Fermentation pathways are helpful to optimize the BHP performance.