The alternative complex III from Rhodothermus marinus – A prototype of a new family of quinol:electron acceptor oxidoreductases

• Published in: FEBS letters Vol. 581; no. 25; pp. 4831 - 4835
• Main Authors: Pereira, Manuela M., Refojo, Patricia N., Hreggvidsson, Gudmundur O., Hjorleifsdottir, Sigridur, Teixeira, Miguel
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 16-10-2007
• Subjects: Amino Acid Sequence; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; bc 1 Complex; bc1 Complex; Complex III; Electron Transport Complex III - chemistry; Electron Transport Complex III - classification; Electron Transport Complex III - genetics; Marine; MFIcc; Molecular Sequence Data; Multigene Family; Oxygen reductase; Protein Subunits - chemistry; Protein Subunits - genetics; Rhodothermus - enzymology; Rhodothermus marinus; bc 1 Complex; MFIcc; Complex III; Oxygen reductase
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2007.09.008

The biochemical and genetic search for a
bc
1 complex in
Rhodothermus marinus was always fruitless; however, a functional equivalent, i.e. having quinol:cytochrome
c oxidoreductase activity was characterized. Now, with the sequencing of
R. marinus genome, it was possible to assign the N-terminal sequences of several proteins of this complex to its coding genes. The alternative complex III from
R. marinus has the same genomic organization of the so-called MFIcc complexes, proposed to be oxidoreductases of the respiratory and photosynthetic electron transfer chains. In this report, we establish undoubtedly the existence of an alternative complex III, a functional substitute of the
bc
1 complex, by its identification at both the biochemical and genomic level.