Effect of neonicotinoid synergists on entomopathogenic nematode fitness

• Published in: Entomologia experimentalis et applicata Vol. 106; no. 1; pp. 7 - 18
• Main Authors: Koppenhöfer, Albrecht M., Cowles, Richard S., Cowles, Elizabeth A., Fuzy, Eugene M., Kaya, Harry K.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-01-2003; Blackwell
• Subjects: Biological and medical sciences; Coleoptera; Control; Cyclocephala; Exomala orientalis; Fundamental and applied biological sciences. Psychology; Heterorhabditis; insect-parasitic nematodes; Integrated pest control; integrated pest management; Phytopathology. Animal pests. Plant and forest protection; Popillia japonica; Protozoa. Invertebrates; scarab; Scarabaeidae; Steinernema; Entomopathogen; Insecticide; Synergist; Coleoptera; Toxicity; Insecta; Biological control; Exomala orientalis; Pest; Beneficial animal; Cydocephala; Nematoda; Integrated pest management; Popillia japonica; Pesticides; Parasite; Steinernema; Guanidines; Heterorhabditis; Arthropoda; Scarabaeidae; Helmintha; Nemathelminthia; Invertebrata; Non target organism; insect-parasitic nematodes; scarab
• ISSN: 0013-8703; 1570-7458
• DOI: 10.1046/j.1570-7458.2003.00008.x

In previous greenhouse and field studies, the neonicotinoid insecticide imidacloprid interacted synergistically with five entomopathogenic nematode species against five scarab species. Two other neonicotinoids, thiamethoxam and acetamiprid, showed a weaker interaction with nematodes in scarab larvae. Entomopathogenic nematodes have the potential to recycle in hosts after inundative applications, thereby increasing the persistence of nematodes and insect control. Thus we investigated the effect of neonicotinoids on nematode fitness after tank mixing and after combined applications. Tank mixing only had a negative effect on nematode survival and infectivity in a few nematode–insecticide combinations and only if both insecticide concentration and exposure time were several times higher than typical for field applications. Combined application of nematodes with imidacloprid generally had no negative effect on the percentage of scarab cadavers producing progeny or the number of nematode progeny emerging per cadaver. In experiments with a synergistic increase in scarab mortality, the total number of progeny in combination treatments was up to four times higher than in nematodes only treatments. Similarly, nematode populations in soil from combination treatments were 13.2 times greater than for nematodes only treatments at 28 days after treatment. Combined imidacloprid–nematode applications did not affect the pathogenicity or infectivity of the nematode progeny. Combining thiamethoxam with nematodes had no negative effects on nematode reproduction in the majority of treatments. However, due to the weaker interaction of thiamethoxam and nematodes on scarab mortality, the total number of nematode progeny per treatment generally did not increase compared with nematodes only treatments. The demonstrated tank mix compatibility of imidacloprid and nematodes improves the feasibility of combining these agents for curative white grub control. The positive effect of imidacloprid on nematode reproduction after combined application may increase the likelihood of infection of white grubs by subsequent generations of nematodes, thereby improving their field persistence and biological control potential.