Interleukin 10 is a Potent Growth and Differentiation Factor for Activated Human B Lymphocytes

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 89; no. 5; pp. 1890 - 1893
• Main Authors: Rousset, Francoise, Garcia, Eric, Defrance, Thierry, Peronne, Catherine, Vezzio, Nadia, Hsu, Di-Hwei, Kastelein, Rob, Moore, Kevin W., Banchereau, Jacques
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01-03-1992; National Acad Sciences; National Academy of Sciences
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Antibody Formation; Antigens; Antigens, CD - physiology; Antigens, Differentiation, B-Lymphocyte - physiology; B lymphocytes; B-Lymphocytes - physiology; Biological and medical sciences; CD40 Antigens; Cell Differentiation; Cell Division; Cell lines; Cells, Cultured; COS cells; Cultured cells; Cytokines; DNA - biosynthesis; Epstein Barr virus infections; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunity (Disease); Immunobiology; Immunoglobulins; In Vitro Techniques; Interleukin-10 - pharmacology; Interleukin-4 - pharmacology; L cells; Lymphocyte Activation; Lymphokines, interleukins ( function, expression); Medical research; Receptors, Antigen, B-Cell - physiology; Recombinant Proteins - pharmacology; Regulatory factors and their cellular receptors; Secretion; Viral Proteins - pharmacology; Human; Cell proliferation; Immunoglobulins; Secretion; Cytokine; Stimulation; Activation; B-Lymphocyte; Cell differentiation; Membrane receptor; Regulation(control); Interleukin 10; Humoral immunity
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.89.5.1890

Interleukin 10 (IL-10), originally identified as a TH2 helper T-cell product able to inhibit cytokine production by TH1 cells, is highly homologous to BCRF1 (viral IL-10), an open reading frame in the Epstein-Barr virus genome. Here, we show that human and viral IL-10 stimulate DNA replication of B lymphocytes activated either via their antigen receptor or via their CD40 antigen. IL-4 and IL-10 display additive effects and induce a strong increase in the number of viable cells. Moreover, IL-10 induces activated B cells to secrete large amounts of IgG, IgA, and IgM, and the combination of IL-10 and IL-4 results in the secretion of the four immunoglobulin isotypes. Thus, IL-10 may play an important role in the amplification of humoral responses.