Broad-Spectrum Cephalosporin-Resistant and/or Fluoroquinolone-Resistant Enterobacterales Associated with Canine and Feline Urogenital Infections

• Published in: Antibiotics (Basel) Vol. 9; no. 7; p. 387
• Main Authors: Loncaric, Igor, Misic, Dusan, Szostak, Michael P, Künzel, Frank, Schäfer-Somi, Sabine, Spergser, Joachim
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07-07-2020; MDPI
• Subjects: Amides; Animals; Antibiotics; Bacteria; Carbapenems; Cats; Cephalosporins; Citrobacter; Cloning; Diseases; E coli; Enterobacter; Enterobacter cloacae; Enterobacterales; Enterobacteriaceae; ESBL; Fluoroquinolones; Genes; Genotyping; Health aspects; infection; Infections; Klebsiella; Loci; Meningitis; Multilocus sequence typing; Mutation; Pandemics; Phylogeny; Prostate; Urogenital system; Veterinarians; Veterinary medicine; Virulence; Virulence factors; β-Lactam antibiotics; Serbia; Austria; AmpC; ST648; infection; Enterobacterales; ST131; ESBL; carbapenem-resistant; urogenital system; ST114; fluoroquinolones; pandemic clone; cephalosporins
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics9070387

The aim of the present study was to characterize
resistant to 3rd and 4th generation cephalosporins, carbapenems and/or fluoroquinolones, isolated from dogs and cats with urogenital infections. In total, 36 strains (
(
= 28),
(
= 3),
,
,
,
and
(each
= 1)) were included in the present study, 28 from Austria and 8 from Serbia. Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and microarray-based assays.
(
)
isolates were additionally characterized by two-locus (
C and
H) sequence phylotyping and multi-locus sequence typing (MLST) of selected isolates. MLST of carbapenem-resistant
isolates was also performed. Among
, the most dominant phylogenetic group was B1 (27.8%), followed by C, (16.6%), A and Clade II (5.5% each), B2 and F (2.77% each). The most predominant β-lactam resistance genes were
(70%) and
(38.8%),
(25%).
was detected in one carbapenem-resistant
ST114. The most common ST among selected
was 744 (10.7% isolates). The pandemic clones ST131 and ST648 carrying CTX-M-15 were also detected. Remaining STs belonged to 469, 1287, 1463 and 1642.
clonotyping revealed 20 CH types. Based on the presence of certain virulence genes, three isolates were categorized as ExPEC/UPEC. The most prevalent virulence factors were
H detected in 61%,
D and
both in 55%,
N in 27.8%,
C in 13.8% and
in 8.3% isolates.