Microbial diversity at Mitchell Peninsula, Eastern Antarctica: a potential biodiversity “hotspot”

• Published in: Polar biology Vol. 39; no. 2; pp. 237 - 249
• Main Authors: Ji, Mukan, van Dorst, Josie, Bissett, Andrew, Brown, Mark V., Palmer, Anne S., Snape, Ian, Siciliano, Steven D., Ferrari, Belinda C.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-02-2016; Springer; Springer Nature B.V
• Subjects: Actinobacteria; Analysis; Ascomycota; Bacteria; Biodiversity; Biological diversity; Biomedical and Life Sciences; Ecology; Firmicutes; Fungi; Life Sciences; Microbiology; Oceanography; Original Paper; Plant Sciences; Proteobacteria; RNA; Soil microbiology; Soils; Studies; Zoology; Windmill Islands; PS, Antarctica; PSE, Antarctica, Wilkes Land, Budd Coast, Windmill Is; Soil; Antarctica; Candidate division; Bacterial diversity; Network analysis; Fungal diversity
• ISSN: 0722-4060; 1432-2056
• DOI: 10.1007/s00300-015-1776-y

Mitchell Peninsula is located towards the East of the Windmill Islands in eastern Antarctica. It is an ice-free polar desert, and knowledge of its soil microbial taxonomic composition is limited. In this study, we investigated the soil microbial taxonomic composition using multiplex 454 pyrosequencing targeting the bacterial 16S rRNA and the fungal ITS genes; and the bacterial and fungal abundances were estimated using quantitative PCR. In total, 40 bacterial and five fungal phyla were identified comprising 111 bacterial and 22 fungal classes, respectively. Mitchell Peninsula soil exhibited a unique bacterial taxonomic profile. In contrast to the usual dominance of Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes in polar and temperate soils, Mitchell Peninsula was rich in the poorly studied Chloroflexi (31.7 %), candidate divisions WPS-2 (8.1 %) and AD3 (5.1 %), while the commonly observed Bacteroidetes and Firmicutes were present in relative abundances below 1 %. The fungal community consisted of Ascomycota (77 %) and Basidiomycota (10 %), and was dominated by the lichenous fungal class Lecanoromycetes (46.4 %). Network analysis revealed the presence of several microbial clusters that each potentially occupied a different environmental niche, and fewer numbers of correlations were identified between bacteria within each cluster compared with the lichen community, where extensive community dynamics may be present. As Mitchell Peninsula exhibits a unique microbial taxonomic composition, not previously observed in any reported polar or temperate ecosystem, we believe it is a potential microbial biodiversity “hotspot”, which warrants further investigation to examine the role of the dominance of these uncharacterised candidate divisions in this extreme ecosystem.