Protective Effects of Houttuynia cordata Thunb. on Gentamicin-induced Oxidative Stress and Nephrotoxicity in Rats

• Published in: Toxicological research (Seoul) Vol. 29; no. 1; pp. 61 - 67
• Main Authors: Kang, C.E., Gyeongnam National University of Science and Technology, Jinju, Republic of Korea, Lee, H.K., Gyeongnam National University of Science and Technology, Jinju, Republic of Korea, Hah, D.Y., Gyeongnam National University of Science and Technology, Jinju, Republic of Korea, Heo, J.H., Gyeongnam National University of Science and Technology, Jinju, Republic of Korea, Kim, C.H., Gyeongnam National University of Science and Technology, Jinju, Republic of Korea, Kim, E.K., Gyeongnam National University of Science and Technology, Jinju, Republic of Korea, Sim, J.S., Gyeongnam National University of Science and Technology, Jinju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Singapore 한국독성학회 01-03-2013; Springer Singapore; The Korean Society of Toxicology
• Subjects: ANTIOXIDANTES; ANTIOXIDANTS; ANTIOXYDANT; Biomedicine; ESPECIES DE OXIGENO REACTIVO; ESTRES OXIDATIVO; Houttuynia cordata Thunb,Gentamicin sulfate,Nephrotoxicity; OXIDATIVE STRESS; Pharmacology/Toxicology; REACTIVE OXYGEN SPECIES; STRESS OXYDATIF; 예방의학; Oxidative stress; Gentamicin sulfate; Houttuynia cordata Thunb; Antioxidant; Nephrotoxicity; Reactive oxygen species (ROS); Thunb; Antioxi-dant
• ISSN: 1976-8257; 2234-2753
• DOI: 10.5487/TR.2013.29.1.061

Development of a therapy providing protection from, or reversing gentamicin-sulfate (GS)-induced oxidative stress and nephrotoxicity would be of great clinical significance. The present study was designed to investigate the protective effects of Houttuynia cordata Thunb. (HC) against gentamicin sulfate-induced renal damage in rats. Twenty-eight Sprague-Dawley rats were divided into 4 equal groups as follows: group 1, control; group 2, GS 100 mg/kg/d, intraperitoneal (i.p.) injection; group 3, GS 100 mg/kg/d, i.p. + HC 500 mg/kg/d, oral; and group 4, GS 100 mg/kg/d i.p. + HC 1000 mg/kg/d, oral administration). Treatments were administered once daily for 12 d. After 12 d, biochemical and histopathological analyses were conducted to evaluate oxidative stress and renal nephrotoxicity. Serum levels of creatinine, malondialdehyde (MDA), and blood urea nitrogen (BUN), together with renal levels of MDA, glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were quantified to evaluate antioxidant activity. Animals treated with GS alone showed a significant increase in serum levels of creatinine, BUN, and MDA, with decreased renal levels of GSH, SOD, and CAT. Treatment of rats with HC showed significant improvement in renal function, presumably as a result of decreased biochemical indices and oxidative stress parameters associated with GS-induced nephrotoxicity. Histopathological examination of the rat kidneys confirmed these observations. Therefore, the novel natural antioxidant HC may protect against GSinduced nephrotoxicity and oxidative stress in rats.