Novel lysophospholipase A secreted by Legionella pneumophila

We show that Legionella pneumophila possesses lysophospholipase A activity, which releases fatty acids from lysophosphatidylcholine. The NH2-terminal sequence of the enzyme contained FGDSLS, corresponding to a catalytic domain in a recently described group of lipolytic enzymes. Culture supernatants...

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Bibliographic Details
Published in:	Journal of bacteriology Vol. 183; no. 6; pp. 2121 - 2124
Main Authors:	Flieger, A, Gong, S, Faigle, M, Stevanovic, S, Cianciotto, N P, Neumeister, B
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 01-03-2001
Series:	Note
Subjects:	Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Cell Surfaces Culture Media Endopeptidases Enzymes Fatty acids Legionella pneumophila Legionella pneumophila - enzymology Legionella pneumophila - genetics Legionella pneumophila - growth & development lysophosphatidylcholine Lysophospholipase - chemistry Lysophospholipase - genetics Lysophospholipase - metabolism Molecular Sequence Data Pathology
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Summary:	We show that Legionella pneumophila possesses lysophospholipase A activity, which releases fatty acids from lysophosphatidylcholine. The NH2-terminal sequence of the enzyme contained FGDSLS, corresponding to a catalytic domain in a recently described group of lipolytic enzymes. Culture supernatants of a L. pneumophila pilD mutant lost the ability to cleave lysophosphatidylcholine.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Department of Microbiology-Immunology, Northwestern University Medical School, Searle 6-573, 320 E Superior St., Chicago, IL 60611. Phone: 001-312-503-1034. Fax: 001-312-503-1339. E-mail: a-flieger@northwestern.edu. Present address: Department of Microbiology and Immunology, Chandler Medical Center, University of Kentucky, Lexington, KY 40536.
ISSN:	0021-9193 1098-5530
DOI:	10.1128/JB.183.6.2121-2124.2001