Interaction of Arcobacter spp. with human and porcine intestinal epithelial cells

• Published in: FEMS immunology and medical microbiology Vol. 50; no. 1; pp. 51 - 58
• Main Authors: Ho, Hoa T.K, Lipman, Len J.A, Hendriks, Henno G.C.J.M, Tooten, Peter C.J, Ultee, Ton, Gaastra, Wim
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-06-2007; Blackwell Publishing Ltd; Blackwell
• Subjects: adhesion; Animals; Arcobacter; Arcobacter - isolation & purification; Arcobacter - pathogenicity; Arcobacter cryaerophilus; Bacterial Adhesion; Biological and medical sciences; Caco-2; Caco-2 Cells; Cell Line; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacterial Infections - immunology; Gram-Negative Bacterial Infections - microbiology; Humans; interleukin-8; Interleukin-8 - biosynthesis; Interleukin-8 - immunology; Intestinal Mucosa - cytology; Intestinal Mucosa - immunology; Intestinal Mucosa - microbiology; invasion; IPI-2I; Microbiology; Sus scrofa; Swine; IPI-2I; adhesion; invasion; interleukin-8; Caco-2; Human; Microbiology; Digestive system; Cytokine; Gut; Adhesion; IPI-21; Vertebrata; Mammalia; Immunology; Swine; Epithelial cell; Artiodactyla; Veterinary; Arcobacter; Ungulata; Interleukin 8
• ISSN: 0928-8244; 1574-695X
• DOI: 10.1111/j.1574-695X.2007.00230.x

Little is known about the pathogenic mechanisms or potential virulence factors of Arcobacter spp. The aim of the study described here was to obtain more insights in the pathogenicity mechanisms of Arcobacter spp. by testing their ability to adhere to, invade and induce interleukin-8 expression in human Caco-2 and porcine IPI-2I cell lines. Eight Arcobacter strains were tested. Four strains were obtained from a culture collection, and represent the four Arcobacter spp. known to be associated with animals and humans. The other four strains were field isolates from the amniotic fluid of sows and from newborn piglets. All eight Arcobacter strains were able to adhere to both cell lines, and induced interleukin-8 production as early as 2 h after a 1h incubation period. This production was still increased 6 h postinfection. Differences in the cell association of the eight strains were obvious, with A. cibarius showing the highest adhesion ability. Invasion of intestinal epithelial cells was only observed for A. cryaerophilus strains. No correlation between invasiveness or strong adhesion of the tested strains and the level of interleukin-8 induction was observed.