Genomic distribution of three repetitive DNAs in cultivated hexaploid Diospyros spp. (D. kaki and D. virginiana) and their wild relatives

• Published in: Genes & Genetic Systems Vol. 78; no. 4; pp. 301 - 308
• Main Authors: Choi, Y.A. (Kyoto Univ. (Japan)), Tao, R, Yonemori, K, Sugiura, A
• Format: Journal Article
• Language: English
• Published: Japan The Genetics Society of Japan 01-08-2003; Japan Science and Technology Agency
• Subjects: Base Sequence; Blotting, Southern; Chromosomes - genetics; Cloning, Molecular; Deoxyribonucleases, Type II Site-Specific - metabolism; DIOSPYROS; Diospyros - genetics; Diospyros glandulosa; Diospyros kaki; Diospyros oleifera; Diospyros virginiana; DNA; DNA Probes; DNA, Plant - genetics; fluorescent in situ hybridization (FISH); GENETIC DISTANCE; Genome, Plant; In Situ Hybridization, Fluorescence; Molecular Sequence Data; Nucleic Acid Hybridization; NUCLEOTIDE SEQUENCE; persimmon; POLYPLOIDY; repetitive DNA; Repetitive Sequences, Nucleic Acid - genetics; RESTRICTION ENZYMES; tandem repeat; WILD PLANTS
• ISSN: 1341-7568; 1880-5779
• DOI: 10.1266/ggs.78.301

To understand the genomic organization of Diospyros species with different ploidy levels, we cloned three different repetitive DNAs and compared their genomic distributions in ten Diospyros species, including hexaploid D. kaki and D. virginiana. Genomic Southern hybridization demonstrated that the EcoRV-repetitive DNA was present in tandem in the genomes of D. glandulosa (2n=2x=30), D. oleifera (2n=2x=30), D. lotus (2n=2x=30), D. virginiana (2n=6x=90) and D. kaki (2n=6x=90). All of these species except D. virginiana also contained the HincII-repetitive DNA in tandem. Fluorescent in situ hybridization showed that the EcoRV- and HincII-repetitive DNAs were predominantly located at the proximal or centromeric regions of chromosomes. The DraI-repetitive sequence cloned from D. ehretioides (2n=2x=30) was not found in the other Diospyros species tested. This suggests that D. ehretioides has a genomic organization different from that of the other Diospyros species. Speciation of hexaploid Diospyros species is also discussed with respect to the genomic distribution of the three repetitive DNAs cloned.