Photogating of ionic currents across lipid bilayers. Electrostatics of ions and dipoles inside the membrane

• Published in: Biophysical journal Vol. 63; no. 6; pp. 1544 - 1555
• Main Authors: Mauzerall, D.C., Drain, C.M.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01-12-1992; Biophysical Society
• Subjects: Artificial membranes and reconstituted systems; Biological and medical sciences; Biophysical Phenomena; Biophysics; conductance; Electric Conductivity; Electrochemistry; Fundamental and applied biological sciences. Psychology; Ion Channel Gating; Ions; ions currents; lipid bilayers; Lipid Bilayers - chemistry; Membrane physicochemistry; Membrane Potentials; Membranes, Artificial; Metalloporphyrins - chemistry; Models, Chemical; Molecular biophysics; Onium Compounds - chemistry; Organophosphorus Compounds - chemistry; Photochemistry; photogating; statistics; Tetraphenylborate - chemistry; tetraphenylboride; tetraphenylphosphonium; Lipophilic compound; Ions; Lipids; Artificial membrane; Membrane potential; Bilayer; Membrane translocation; Electrostatic model
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/S0006-3495(92)81738-1

The conductances of the lipophilic ions tetraphenylboride and tetraphenylphosphonium across a lipid bilayer can be increased or decreased, i.e., gated, by the photoformation of closed-shell metalloporphyrin cations within the bilayer. The gating can be effected by pulsed or continuous light or by chemical oxidants. At high concentrations of lipophilic anions where the dark conductance is saturated due to space charge in the bilayer, the photogated conductance can increase 15-fold. The formation of porphyrin cations allows the conductance to increase to its nonspace charge limited value. Conversely, the decrease of conductance in the light of phosphonium cations diminishes toward zero as the dark conductance becomes space charge limited. We present electrostatic models of the space charge limited conductance that accurately fit the data. One model includes an exponentially varying dielectric constant for the polar regions of the bilayer that allows an analytical solution to the electrostatic problem. The exponential variation of the dielectric constant effectively screens the potential and implies that the inside and outside of real dielectric interfaces can be electrically isolated from one another. The charge density, the distance into the membrane of the ions, about one-quarter of its thickness, and the dielectric constant at that position are determined by these models. These calculations indicate that there is insufficient porphyrin charge density to cancel the boride ion space charge and the following article proposes a novel ion chain mechanism to explain these effects. These models indicate that the positive potential arising from oriented carbonyl ester groups, previously used to explain the 10(3)-fold larger conductance of hydrophobic anions over cations, is smaller than previously estimated. However, the synergistic movement of the positive choline group into the membrane can account for the large positive potential.