Extracellular matrix protein composition dynamically changes during murine forelimb development

• Published in: iScience Vol. 27; no. 2; p. 108838
• Main Authors: Jacobson, Kathryn R., Saleh, Aya M., Lipp, Sarah N., Tian, Chengzhe, Watson, Audrey R., Luetkemeyer, Callan M., Ocken, Alexander R., Spencer, Sabrina L., Kinzer-Ursem, Tamara L., Calve, Sarah
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16-02-2024; Elsevier
• Subjects: Biological sciences; Developmental biology; Natural sciences; Natural sciences; Developmental biology; Biological sciences
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2024.108838

The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5–14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development.
[Display omitted]
•ECM composition changed during forelimb development, distinct from other organs•ECM protein dynamics and organization cannot be predicted by transcriptomics•In vivo metabolic labeling identified dynamics of nascent ECM in forelimbs
Natural sciences; Biological sciences; Developmental biology