Distribution and biological role of the oligopeptide-binding protein (OppA) in Xanthomonas species

• Published in: Genetics and molecular biology Vol. 33; no. 2; pp. 341 - 347
• Main Authors: Oshiro, Elisa E, Tavares, Milene B, Suzuki, Celso F, Pimenta, Daniel C, Angeli, Claudia B, de Oliveira, Julio C F, Ferro, Maria I T, Ferreira, Luis C S, Ferreira, Rita C C
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira de Genética 01-01-2010
• Subjects: ABC transporter; BIOCHEMISTRY & MOLECULAR BIOLOGY; GENETICS & HEREDITY; Genetics of Microorganisms; oligopeptide uptake system; OppA; Xac; Xanthomonas; Xac; oligopeptide uptake system; ABC transporter; OppA; Xanthomonas
• ISSN: 1415-4757; 1678-4685; 1678-4685
• DOI: 10.1590/S1415-47572010005000049

In this study we investigated the prevalence of the oppA gene, encoding the oligopeptide binding protein (OppA) of the major bacterial oligopeptide uptake system (Opp), in different species of the genus Xanthomonas. The oppA gene was detected in two Xanthomonas axonopodis strains among eight tested Xanthomonas species. The generation of an isogenic oppA-knockout derivative of the Xac 306 strain, showed that the OppA protein neither plays a relevant role in oligopeptide uptake nor contributes to the infectivity and multiplication of the bacterial strain in leaves of sweet orange (Citrus sinensis) and Rangpur lime (Citrus limonia). Taken together these results suggest that the oppA gene has a recent evolutionary history in the genus and does not contribute in the physiology or pathogenesis of X. axonopodis.