2-Aminobenzothiazole degradation by free and Ca-alginate immobilized cells of Rhodococcus rhodochrous

• Published in: Chemosphere (Oxford) Vol. 75; no. 1; pp. 121 - 128
• Main Authors: Chorao, Charlène, Charmantray, Franck, Besse-Hoggan, Pascale, Sancelme, Martine, Cincilei, Angela, Traïkia, Mounir, Mailhot, Gilles, Delort, Anne-Marie
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-03-2009; Elsevier
• Subjects: 31P NMR; Alginates - chemistry; Analytical chemistry; Animal, plant and microbial ecology; Applied ecology; Benzothiazole; Benzothiazoles - metabolism; Biodegradation; Biodegradation of pollutants; Biological and medical sciences; Biotechnology; Biotransformation; Cells, Immobilized - metabolism; Chemical Sciences; Chromatography, Liquid; Ecotoxicology, biological effects of pollution; Environment and pollution; FeNTA; Ferric Compounds - chemistry; Fundamental and applied biological sciences. Psychology; General aspects; Glucuronic Acid - chemistry; Hexuronic Acids - chemistry; Industrial applications and implications. Economical aspects; Light; Magnetic Resonance Spectroscopy; Nitrilotriacetic Acid - analogs & derivatives; Nitrilotriacetic Acid - chemistry; Photodegradation; Photolysis; Rhodococcus - chemistry; Rhodococcus - metabolism; Rhodococcus rhodochrous; Time Factors; Photodegradation; Biodegradation; FeNTA; Benzothiazole; 31P NMR; Rhodococcus rhodochrous; Pollutant behavior; Bacteria; Actinomycetes; Immobilized cell; Nuclear magnetic resonance; Photochemical degradation
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2008.11.021

2-Aminobenzothiazole (ABT) degradation was investigated using free and immobilized systems during photodegradation under solar light in the presence of Fe(III)-nitrilotriacetic acid (FeNTA), biodegradation by
Rhodococcus rhodochrous, and during combined conditions. Ca-alginate hydrogel was chosen as a model matrix and some complementary studies were required to characterize this new system.
R. rhodochrous metabolism in this type of environment was monitored by NMR spectroscopy. Neither change in intracellular pH values nor in ATP concentrations was observed by
in vivo
31P NMR, showing that no metabolic modification occurred between free and immobilized cells.
1H NMR demonstrated that alginate was not used as carbon source by
R. rhodochrous. After establishing the pre-treatment protocol by SPE to eliminate solubilised alginate, ABT adsorption on beads and degradation were studied. The same pathways of transformation were observed in suspended and immobilized cell systems. Considering the ABT adsorption phenomenon on alginate beads (8%), the efficiency of the two systems was found to be comparable although the degradation rate was slightly lower with immobilized cells. The most important result was the finding that the positive effect of FeNTA on ABT degradation with immobilized cells was similar to that observed previously with free cells. All these results show that mechanisms observed with free cells can be extrapolated to entrapped cells, i.e. under conditions much closer to those usually encountered in the environment.