Cold Plasma Controls Nitrite Hazards by Modulating Microbial Communities in Pickled Radish

• Published in: Foods Vol. 12; no. 13; p. 2550
• Main Authors: Wei, Wei, Yang, Shujing, Yang, Fan, Hu, Xinyu, Wang, Yuan, Guo, Wenjun, Yang, Biyue, Xiao, Xiang, Zhu, Lin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29-06-2023; MDPI
• Subjects: Acid production; Additives; Bacteria; Cold; cold plasma; Cold plasmas; Control methods; Degradation; Dielectric barrier discharge; Energy consumption; Fermentation; Food; Food safety; Food science; Gram-negative bacteria; Gram-positive bacteria; Hazards; Lactic acid; Lactic acid bacteria; Microbial activity; Microbiomes; Microorganisms; Next-generation sequencing; Nitrates; nitrite; Nitrites; pickled radish; Plasma; Radishes; Raw materials; Safety and security measures; Sterilization; Vegetables; China; pickled radish; nitrite; Gram-negative bacteria; cold plasma; lactic acid bacteria
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12132550

The hazard of nitrite caused by microorganisms is the main food safety problem in the pickle production. To seek a method to control the nitrite hazards of pickles by regulating microbial community without additional substances, we focused on cold plasma because Gram-negative and Gram-positive bacteria have different degrees of sensitivity to the sterilization of cold plasma. Using radish pickles as the experimental object, based on colony counting, dynamic monitoring of pH and nitrite, qPCR and high-throughput sequencing, it was found that when the raw material was treated with dielectric barrier discharge (DBD) cold plasma at 40 kV for 60 s, Gram-negative bacteria with the potential to produce nitrite were preferentially sterilized. Meanwhile, Gram-positive bacteria dominated by the lactic acid bacteria were retained to accelerate the acid production rate, initiate the self-degradation of nitrite in advance and significantly reduce the peak value and accumulation of nitrite during the fermentation process of pickled radish. This study preliminarily verified that DBD cold plasma can inhibit the nitrite generation and accelerate the self-degradation of nitrite by regulating the structure and abundance of microbial community in radish pickles, which provides an important reference for the control of nitrite hazards in the fermentation process of pickles without additives.