Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning

Here, we describe a protocol to identify escape mutants on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) receptor-binding domain (RBD) using a yeast screen combined with deep mutational scanning. Over 90% of all potential single S RBD escape mutants can be identified for...

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Bibliographic Details
Published in:	STAR protocols Vol. 2; no. 4; p. 100869
Main Authors:	Haas, Cyrus M., Francino-Urdaniz, Irene M., Steiner, Paul J., Whitehead, Timothy A.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 17-12-2021 Elsevier
Subjects:	Angiotensin-Converting Enzyme 2 - genetics Angiotensin-Converting Enzyme 2 - metabolism Antibody Binding Sites COVID-19 - genetics COVID-19 - metabolism COVID-19 - virology DNA Mutational Analysis - methods Humans Immunology Microbiology Molecular Biology Mutation Protein Biochemistry Protocol Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism SARS-CoV-2 - genetics Sequence analysis Sequencing Spike Glycoprotein, Coronavirus - genetics Spike Glycoprotein, Coronavirus - metabolism Protein Biochemistry Immunology Microbiology Molecular Biology Antibody Sequence analysis Sequencing
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