Assessing the In Vivo Biocompatibility of Molecularly Imprinted Polymer Nanoparticles

Molecularly imprinted polymer nanoparticles (nanoMIPs) are high affinity synthetic receptors which show promise as imaging and therapeutic agents. Comprehensive analysis of the in vivo behaviour of nanoMIPs must be performed before they can be considered for clinical applications. This work reports...

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Bibliographic Details
Published in:	Polymers Vol. 14; no. 21; p. 4582
Main Authors:	Kassem, Samr, Piletsky, Stanislav S, Yesilkaya, Hasan, Gazioglu, Ozcan, Habtom, Medhanie, Canfarotta, Francesco, Piletska, Elena, Spivey, Alan C, Aboagye, Eric O, Piletsky, Sergey A
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 28-10-2022 MDPI
Subjects:	Antibodies Biocompatibility Biodistribution Blood-brain barrier Book publishing Brain research Chemical compounds cytotoxicity Drug dosages Health aspects Imprinted polymers Laboratory animals Liver molecularly imprinted polymers Nanomaterials Nanoparticles Peptides Permeability Pharmacology Polymers Proteins Solid phase synthesis Spleen Toxicity United Kingdom United Kingdom > UK biodistribution blood–brain barrier nanoparticles cytotoxicity molecularly imprinted polymers
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Summary:	Molecularly imprinted polymer nanoparticles (nanoMIPs) are high affinity synthetic receptors which show promise as imaging and therapeutic agents. Comprehensive analysis of the in vivo behaviour of nanoMIPs must be performed before they can be considered for clinical applications. This work reports the solid-phase synthesis of nanoMIPs and an investigation of their biodistribution, clearance and cytotoxicity in a rat model following both intravenous and oral administration. These nanoMIPs were found in each harvested tissue type, including brain tissue, implying their ability to cross the blood-brain barrier. The nanoMIPs were cleared from the body via both faeces and urine. Furthermore, we describe an immunogenicity study in mice, demonstrating that nanoMIPs specific for a cell surface protein showed moderate adjuvant properties, whilst those imprinted for a scrambled peptide showed no such behaviour. Given their ability to access all tissue types and their relatively low cytotoxicity, these results pave the way for in vivo applications of nanoMIPs.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2073-4360 2073-4360
DOI:	10.3390/polym14214582