Quantification of 2,4-D on Solid-Phase Exposure Sampling Media by LC-MS-MS

• Published in: Journal of analytical toxicology Vol. 29; no. 3; pp. 188 - 192
• Main Authors: Gardner, M., Spruill-McCombs, M., Beach, J., Michael, L., Thomas, K., Helburn, R.S.
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01-04-2005; Preston
• Subjects: 2,4-Dichlorophenoxyacetic Acid - analysis; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid; Herbicides - analysis; Humans; Mass Spectrometry; Medical sciences; Occupational Exposure - analysis; Pesticides, fertilizers and other agrochemicals toxicology; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Specimen Handling; Toxicology; Trace analysis; Validation; Solid phase extraction; Chemical analysis; 2,4-D; Coupled method; Pesticides; Cellulose; HPLC chromatography; Systemic; Electrospray; Herbicide; Chemical enrichment; Sample preparation; Mass spectrometry MS/MS; Polyurethane; Crosslinked polymer; Styrene polymer; Phytohormone; Adsorbent; Sampling; Quantitative analysis
• ISSN: 0146-4760; 1945-2403
• DOI: 10.1093/jat/29.3.188

Three types of solid-phase chemical exposure sampling media: cellulose, polyurethane foam, and XAD-2, were analyzed for 2,4-dichlorophenoxyacetic acid (2,4-D) and the amine salts of 2,4-D. Individual samples were extracted into acidified methanol, and the extracts were analyzed via liquid chromatography-tandem mass spectrometry using electrospray ionization and a C8 reversed-phase LC column. 13C 2,4-D and a labeled benzoic acid (13C or d5) were employed as the surrogate and internal standard, respectively. Over a three-year study of pesticide exposure among agricultural workers involving a high volume of analyses, values of average % recovery for 13C 2,4-D on personal exposure samples ranged from 87 to 98 with a % relative standard deviation ranging from 7 to 12. The performance of the method compares favorably with that of the current National Institute for Occupational Safety and Health-recommended approach for the analysis of 2,4-D on these types of exposure sampling matrices.