Contribution of the different omega-3 fatty acid desaturase genes to the cold response in soybean

• Published in: Journal of experimental botany Vol. 63; no. 13; pp. 4973 - 4982
• Main Authors: Román, Ángela, Andreu, Vanesa, Hernández, María Luisa, Lagunas, Beatriz, Picorel, Rafael, Martínez-Rivas, José Manuel, Alfonso, Miguel
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press [etc.] 01-08-2012; OXFORD UNIVERSITY PRESS; Oxford University Press
• Subjects: Acclimatization - genetics; alpha-Linolenic Acid - analysis; alpha-Linolenic Acid - metabolism; Alternative Splicing; Amino Acid Sequence; Biological and medical sciences; Cell Culture Techniques; cold; cold stress; Cold Temperature; endoplasmic reticulum; Endoplasmic Reticulum - enzymology; Fatty Acid Desaturases - genetics; Fatty Acid Desaturases - metabolism; Fatty acids; Fatty Acids - analysis; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant - genetics; Genes; Glycine max - enzymology; Glycine max - genetics; Glycine max - physiology; Hydroponics; Isoenzymes; Leaves; linolenic acid; Lipids; Low temperature; messenger RNA; Molecular Sequence Data; omega-3 fatty acids; Plant Leaves - enzymology; Plant Leaves - genetics; Plant Leaves - physiology; Plant physiology and development; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Plastids - enzymology; Protein isoforms; RESEARCH PAPER; RNA, Messenger - genetics; RNA, Plant - genetics; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Sequence Alignment; Soybeans; stearoyl-CoA desaturase; temperature; Temperature control; Yeasts; Cold; Enzyme; FAD3; Soybean; Gene expression; Glycine max; FAD7; FAD8; Grain legume; Leguminosae; Gene; Dicotyledones; Angiospermae; Botany; Spermatophyta; desaturase; Oxidoreductases
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/ers174

This study analysed the contribution of each omega-3 desaturase to the cold response in soybean. Exposure to cold temperatures (5 °C) did not result in great modifications of the linolenic acid content in leaf membrane lipids. However, an increase in the GmFAD3A transcripts was observed both in plant leaves and soybean cells whereas no changes in GmFAD3B or GmFAD3C expression levels were detected. This increase was reversible and accompanied by the accumulation of an mRNA encoding a truncated form of GmFAD3A (GmFAD3A-T), which originated from alternative splicing of GmFAD3A in response to cold. When the expression of plastidial omega-3 desaturases was analysed, a transient accumulation of GmFAD7-2 mRNA was detected upon cold exposure in mature soybean trifoliate leaves while GmFAD7-1 transcripts remained unchanged. No modification of the GmFAD8-1 and GmFAD8-2 transcripts was observed. The functionality of GmFAD3A, GmFAD3B, GmFAD3C and GmFAD3A-T was examined by heterologous expression in yeast. No activity was detected with GmFAD3A-T, consistent with the absence of one of the His boxes necessary for desaturase activity. The linolenic acid content of Sacharomyces cerevisiae cells overexpressing GmFAD3A or GmFAD3B was higher when the cultures were incubated at cooler temperatures, suggesting that reticular desaturases of the GmFAD3 family, and more specifically GmFAD3A, may play a role in the cold response, even in leaves. The data point to a regulatory mechanism of omega-3 fatty acid desaturases in soybean affecting specific isoforms in both the plastid and the endoplasmic reticulum to maintain appropriate levels of linolenic acid under low temperature conditions.