Preparation of molecular imprinted film based on chitosan/nafion/nano-silver/poly quercetin for clenbuterol sensing

• Published in: Food chemistry Vol. 129; no. 2; pp. 595 - 600
• Main Authors: Zhao, Chang, Jin, Guan-Ping, Chen, Li-Li, Li, Yi, Yu, Bo
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15-11-2011; Elsevier
• Subjects: Biological and medical sciences; Chitosan; Clenbuterol; Detection; detection limit; Electrodes; Field emission; Food industries; Fundamental and applied biological sciences. Psychology; liver; Molecular imprinting polymer; Nano-silver; Nanocomposites; Nanomaterials; Nanostructure; pork; quercetin; scanning electron microscopy; X-ray photoelectron spectroscopy; Clenbuterol; Nano-silver; Chitosan; Molecular imprinting polymer; Silver; Polyphenol; Film; Imprinting; Preparation; Quercetin; Polymer; Flavonoid
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2011.04.072

► Nano-Ag could catalyse the redox of clenbuterol. ► Molecular imprinted film consisted of biopolymer chitosan, inorganic nano-Ag and poly quercetin. ► Electrochemical preparation of the molecular imprinted film. ► Clenbuterol determination in practical samples at the molecular imprinted film.
A useful molecular imprinted film (MIP-CNSQ/WGE) based on chitosan/nafion/nano-silver/poly quercetin compound was prepared by a compound electrochemical method at paraffin-impregnated graphite electrode for clenbuterol (CLB) sensing, which was characterised by means of field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy spectra (XPS), infrared spectra (IR) and electrochemical techniques to confirm the formation. The molecular imprinted film modified electrode was successfully applied to the determination of CLB with a reliable result. In optimum conditions, CLB at concentrations of 0.3–50.0μM could be determined with a detection limit of 0.01μM (3σ). Determination of CLB in practical samples of pork liver showed good recovery.