Association of HLA-G01:01:02:01/G01:04:01 polymorphism with gastric adenocarcinoma

• Published in: Human immunology Vol. 77; no. 2; pp. 153 - 157
• Main Authors: Khorrami, Samaneh, Rahimi, Roghayeh, Mohammadpour, Hemn, Bahrami, Salahadin, Yari, Fatemeh, Poustchi, Hossein, Malekzadeh, Reza
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-02-2016
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - immunology; Adult; Aged; Aged, 80 and over; Allergy and Immunology; Case-Control Studies; Female; Gastric adenocarcinoma; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; HLA-G; HLA-G Antigens - genetics; Humans; Iran; Iranian population; Male; Middle Aged; Polymorphism; Polymorphism, Genetic; Stomach Neoplasms - genetics; Stomach Neoplasms - immunology; Tumor Escape - genetics; Gastric adenocarcinoma; Iranian population; HLA-G; Polymorphism
• ISSN: 0198-8859; 1879-1166
• DOI: 10.1016/j.humimm.2015.11.005

Abstract Human leukocyte antigen-G (HLA-G) plays an important role in tumor cell escape from immune surveillance and HLA-G polymorphisms might service as a potential risk factor for clinical outcomes in GAC (gastric adenocarcinoma). We investigated the association between HLA-G polymorphisms as well as soluble HLA-G level and accordance of GAC. This case-control study included 100 GAC patients and 102 unrelated Iranian individual’s samples as control. The clinical stages ranged from I to IV. PCR-RFLP method was carried out in order to specify the genotypes of the HLA-G gene. Concentrations of sHLA-G in serum were determined with the sHLA-G-specific enzyme linked immunosorbent assay (ELISA) kit. The G*01:04:01 and G*01:01:02:01 alleles were the predominant alleles in GAC patients and healthy controls. The G*01:01:03:01 and G*01:01:08 allele distributions are significantly higher among controls comparing to cases and seem to have protective effect ( P value = 0.026 and 0.007 respectively). There is a substantial differences in G*01:01:02:01/G*01:04:01 genotype frequencies between cases and controls (OR = 2.8, P value < 0.001). The G*01:01:03:01/G*01:04:01 and G*01:01:02:01/G*01:01:08 genotypes frequency are higher among controls in comparison to patients ( P value = 0.028 and 0.007 respectively). The polymorphisms in HLA-G could affect GAC induction and its outcome. Also, increased sHLA-G levels in serum might be a useful biomarker for diagnosis.