Fine mapping and validation of a major QTL for grain weight on chromosome 5B in bread wheat

• Published in: Theoretical and applied genetics Vol. 134; no. 11; pp. 3731 - 3741
• Main Authors: Zhao, Dehui, Yang, Li, Liu, Dan, Zeng, Jianqi, Cao, Shuanghe, Xia, Xianchun, Yan, Jun, Song, Xiyue, He, Zhonghu, Zhang, Yong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-11-2021; Springer; Springer Nature B.V
• Subjects: Agriculture; Analysis; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; Chromosomes; Chromosomes, Plant; Cloning; Crop yields; Cultivars; Edible Grain - genetics; Gene mapping; Genes; Genomes; Genomics; Grain; Grain size; Life Sciences; Molecular modelling; Original Article; Phenotype; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Population; Quantitative genetics; Quantitative Trait Loci; Seasons; Seeds; Seeds - growth & development; Single-nucleotide polymorphism; Triticum - genetics; Wheat; Yang, Li; South Korea; China
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-021-03925-9

Key message
A major QTL QTgw.caas-5B for thousand grain weight in wheat was fine mapped on chromosome 5B, and TraesCS5B02G044800 was predicted to be the candidate gene.
Thousand grain weight (TGW), determined by grain length and width, and is an important yield component in wheat; understanding of the underlying genes and molecular mechanisms remains limited. A stable QTL
QTgw.caas-5B
for TGW was identified previously in a RIL population developed from a cross between Zhongmai 871 (ZM871) and a sister line Zhongmai 895 (ZM895), and the aim of this study was to perform fine mapping and validate the genetic effect of the QTL. It was delimited to an interval of approximately 2.0 Mb flanked by markers
Kasp_5B29
and
Kasp_5B31
(49.6–51.6 Mb) using 12 heterozygous recombinant plants obtained by selfing a residual BC
1
F
6
line selected from the ZM871/ZM895//ZM871 population. A candidate gene was predicted following sequencing and differential expression analyses. Marker
Kasp_5B_Tgw
based on a SNP in
TraesCS5B02G044800
, the
QTgw.caas-5B
candidate, was developed and validated in a diversity panel of 166 cultivars. The precise mapping of
QTgw.caas-5B
laid a foundation for cloning of a predicted causal gene and provides a molecular marker for improving grain yield in wheat.