Over-Expression of the Testis-Specific Gene TSGA10 in Cancers and Its Immunogenicity

• Published in: Microbiology and immunology Vol. 48; no. 4; pp. 339 - 345
• Main Authors: Tanaka, Ryo, Ono, Toshiro, Sato, Shuichiro, Nakada, Tetsuya, Koizumi, Fumihito, Hasegawa, Kosei, Nakagawa, Kazuhiko, Okumura, Hideo, Yamashita, Toshiharu, Ohtsuka, Mikio, Asagoe, Kenji, Yamasaki, Osamu, Noguchi, Yuji, Iwatsuki, Keiji, Nakayama, Eiichi
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01-01-2004
• Subjects: Antibodies - blood; cancer; Humans; Male; Neoplasms - immunology; Neoplasms - metabolism; over-expression; Proteins - genetics; Proteins - immunology; Proteins - metabolism; real-time RT-PCR; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Testis; TSGA10; Up-Regulation
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.2004.tb03515.x

The TSGA10 gene was originally isolated in normal testis by differential mRNA display. TSGA10 is located on chromosome 2q11.2 and consists of 19 exons extending over 3 kb. TSGA10 mRNA expression was investigated in normal and malignant tissues using quantitative real‐time RT‐PCR. It was predominantly expressed in the testis in adult normal tissues. In malignant tissues, TSGA10 was over‐expressed in 4 of 20 hepatocellular carcinomas (HCC), 1 of 20 colon cancers, 7 of 20 ovarian cancers, 3 of 20 prostate cancers, 1 of 21 malignant melanomas, and 8 of 21 bladder cancers. Serological analysis revealed that 3 out of 346 patients with various types of cancer possessed antibody against recombinant TSGA10 protein. They included 2 patients with hepatocellular carcinoma and a patient with malignant melanoma.