CircRNA8220 Sponges MiR-8516 to Regulate Cell Viability and Milk Synthesis via Ras/MEK/ERK and PI3K/AKT/mTOR Pathways in Goat Mammary Epithelial Cells

• Published in: Animals (Basel) Vol. 10; no. 8; p. 1347
• Main Authors: Zhu, Chao, Jiang, Yue, Zhu, Junru, He, Yonglong, Yin, Hao, Duan, Quyu, Zhang, Lei, Cao, Binyun, An, Xiaopeng
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-08-2020; MDPI
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Apoptosis; Cancer; Casein; Cell culture; Cell viability; circRNA8220; Epithelial cells; Extracellular signal-regulated kinase; Gene expression; Genetic aspects; Goats; Health aspects; Mammary gland; MicroRNA; Milk; Milk production; miR-8516; Molecular modelling; Negative feedback; Penicillin; Plasmids; Proteins; Reagents; Regulators; Stanniocalcin; STC2; TOR protein; Triglycerides; β-casein; China; Beijing China; United States > US
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani10081347

Circular RNAs (circRNAs), which are considered a large class of endogenous noncoding RNAs, function as regulators in various biological procedures. In this study, the function and molecular mechanisms of circRNA8220 in goat mammary epithelial cells (GMECs) were explored. CircRNA8220 could spong miR-8516 and block the function of miR-8516 by binding to the target site of miR-8516 a negative feedback relationship existed between circRNA8220 and miR-8516. Stanniocalcin 2 (STC2) was a target gene of miR-8516. circRNA8220 could up-regulate the expression of STC2 by sponging miR-8516 in GMECs. circRNA8220/miR-8516/STC2 could promote proliferation and enhance the synthesis of β-casein and triglycerides (TG) via Ras/MEK/ERK and PI3K/AKT/mTOR signaling pathways, respectively.