Transcriptomic analysis of wheat reveals possible resistance mechanism mediated by Yr10 to stripe rust

• Published in: Stress biology Vol. 3; no. 1; p. 44
• Main Authors: Wu, Zhongyi, Zhang, Gaohua, Zhao, Ran, Gao, Qi, Zhao, Jinchen, Zhu, Xiaoxu, Wang, Fangyan, Kang, Zhensheng, Wang, Xiaojing
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 23-10-2023; Springer Nature B.V; Springer
• Subjects: (VIGS) system; 4-hydroxyphenylpyruvate dioxygenase; Animal Physiology; Biomedical and Life Sciences; Cluster analysis; Genes; Genomes; HPPD; Infections; Life Sciences; Microbiology; Original Paper; Pathogens; Plant diseases; Plant resistance; Plant Sciences; Transcriptome; Virus-induced gene silencing system; Yr10; (VIGS) system; HPPD; Transcriptome; Virus-induced gene silencing system
• ISSN: 2731-0450; 2731-0450
• DOI: 10.1007/s44154-023-00115-z

Stripe rust, caused by
Puccinia striiformis
f. sp.
tritici
(
Pst
), is a catastrophic disease that threatens global wheat yield.
Yr10
is a race-specific all-stage disease resistance gene in wheat. However, the resistance mechanism of
Yr10
is poorly characterized. Therefore, to elucidate the potential molecular mechanism mediated by
Yr10
, transcriptomic sequencing was performed at 0, 18, and 48 h post-inoculation (hpi) of compatible wheat Avocet S (AvS) and incompatible near-isogenic line (NIL) AvS + 
Yr10
inoculated with
Pst
race CYR32. Respectively, 227, 208, and 4050 differentially expressed genes (DEGs) were identified at 0, 18, and 48 hpi between incompatible and compatible interaction. The response of
Yr10
to stripe rust involved various processes and activities, as indicated by the results of Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Specifically, the response included photosynthesis, defense response to fungus, metabolic processes related to salicylic acid (SA) and jasmonic acid (JA), and activities related to reactive oxygen species (ROS). Ten candidate genes were selected for qRT-PCR verification and the results showed that the transcriptomic data was reliable. Through the functional analysis of candidate genes by the virus-induced gene silencing (VIGS) system, it was found that the gene
TaHPPD
(
4-hydroxyphenylpyruvate dioxygenase
) negatively regulated the resistance of wheat to stripe rust by affecting SA signaling,
pathogenesis-related
(
PR
) gene expression, and ROS clearance. Our study provides insight into
Yr10-
mediated resistance in wheat.