Spliceosomal component Sf3b1 is essential for hematopoietic differentiation in zebrafish

• Published in: Experimental hematology Vol. 44; no. 9; pp. 826 - 837.e4
• Main Authors: De La Garza, Adriana, Cameron, Rosannah C, Nik, Sara, Payne, Sara G, Bowman, Teresa V
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-09-2016
• Subjects: Advanced Basic Science; Animals; Apoptosis - genetics; Biomarkers; Cell Differentiation - genetics; Erythroid Cells - cytology; Erythroid Cells - metabolism; Hematology, Oncology and Palliative Medicine; Hematopoiesis - genetics; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - metabolism; Mutation; Myeloid Cells - cytology; Myeloid Cells - metabolism; Receptors, Notch - metabolism; RNA Splicing Factors - genetics; Signal Transduction; Zebrafish - genetics; Zebrafish - metabolism; Zebrafish Proteins - genetics
• ISSN: 0301-472X; 1873-2399
• DOI: 10.1016/j.exphem.2016.05.012

SF3B1 ( Splicing factor 3b , subunit 1 ) is one of the most commonly mutated factors in myelodysplastic syndrome (MDS). Although the genetic correlation between SF3B1 mutations and MDS etiology are quite strong, no in vivo model currently exists to explore how SF3B1 loss alters blood cell development. Using zebrafish mutants, we show here that proper function of Sf3b1 is required for all hematopoietic lineages. As in MDS patients, zebrafish sf3b1 mutants develop a macrocytic-anemia–like phenotype due to a block in maturation at a late progenitor stage. The mutant embryos also develop neutropenia, because their primitive myeloid cells fail to mature and turn on differentiation markers such as l-plastin and myeloperoxidase. In contrast, production of definitive hematopoietic stem and progenitor cells (HSPCs) from hemogenic endothelial cells within the dorsal aorta is greatly diminished, whereas arterial endothelial cells are correctly fated. Notch signaling, imperative for the endothelial-to-hematopoietic transition, is also normal, indicating that HSPC induction is blocked in sf3b1 mutants downstream or independent of Notch signaling. The data demonstrate that Sf3b1 function is necessary during key differentiation fate decisions in multiple blood cell types. Zebrafish sf3b1 mutants offer a novel animal model with which to explore the role of splicing in hematopoietic development and provide an excellent in vivo system with which to delve into the question of why and how Sf3b1 dysfunction is detrimental to hematopoietic differentiation, which could improve MDS diagnosis and treatment.