Porous titanium and Ti–35Nb alloy: effects on gene expression of osteoblastic cells derived from human alveolar bone

• Published in: Journal of materials science. Materials in medicine Vol. 26; no. 11; pp. 259 - 11
• Main Authors: do Prado, Renata Falchete, Rabêlo, Sylvia Bicalho, de Andrade, Dennia Perez, Nascimento, Rodrigo Dias, Henriques, Vinicius André Rodrigues, Carvalho, Yasmin Rodarte, Cairo, Carlos Alberto Alves, de Vasconcellos, Luana Marotta Reis
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-11-2015; Springer Nature B.V
• Subjects: Adult; Alloys; Alveolar Process - cytology; Alveolar Process - metabolism; Biocompatibility; Biomaterials; Biomedical engineering; Biomedical Engineering and Bioengineering; Biomedical materials; Bones; Ceramics; Chemistry and Materials Science; Composites; Female; Flowers & plants; Gene Expression; Glass; Human; Humans; Male; Materials Science; Microscopy, Electron, Scanning; Natural Materials; Niobium; Osteoblasts - metabolism; Polymer Sciences; Porosity; Porous materials; Real-Time Polymerase Chain Reaction; Regenerative Medicine/Tissue Engineering; Surfaces and Interfaces; Surgical implants; Thin Films; Tissue Engineering Constructs and Cell Substrates; Tissue Scaffolds; Titanium; Titanium base alloys; X-Ray Diffraction; Porous Titanium; Osteocalcin; Porous Sample; Human Osteoblastic Cell; 35Nb Alloy
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-015-5594-0

Tests on titanium alloys that possess low elastic modulus, corrosion resistance and minimal potential toxicity are ongoing. This study aimed to evaluate the behavior of human osteoblastic cells cultured on dense and porous Titanium (Ti) samples comparing to dense and porous Ti
–
35 Niobium (Ti–35Nb) samples, using gene expression analysis. Scanning electronic microscopy confirmed surface porosity and pore interconnectivity and X-ray diffraction showed titanium beta-phase stabilization in Ti–35Nb alloy. There were no differences in expression of transforming growth factor-β, integrin-β1, alkaline phosphatase, osteopontin, macrophage colony stimulating factor, prostaglandin E synthase, and apolipoprotein E regarding the type of alloy, porosity and experimental period. The experimental period was a significant factor for the markers: bone sialoprotein II and interleukin 6, with expression increasing over time. Porosity diminished Runt-related transcription factor-2 (Runx-2) expression. Cells adhering to the Ti–35Nb alloy showed statistically similar expression to those adhering to commercially pure Ti grade II, for all the markers tested. In conclusion, the molecular mechanisms of interaction between human osteoblasts and the Ti–35Nb alloy follow the principal routes of osseointegration of commercially pure Ti grade II. Porosity impaired the route of transcription factor Runx-2.