Immuno-fluorescence staining patterns of leukocyte subsets in the skin of taurine and indicine cattle

• Published in: Research in veterinary science Vol. 95; no. 3; pp. 854 - 860
• Main Authors: Constantinoiu, C.C., Jonsson, N.N., Jorgensen, W.K., Jackson, L.A., Piper, E.K., Lew-Tabor, A.E.
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01-12-2013; Elsevier Limited
• Subjects: Animals; antibodies; Antibodies, Monoclonal - immunology; Brahman; Breed; Cattle; Cold; Cytotoxicity; Epitopes - immunology; Fluorescent Antibody Technique - veterinary; granulocytes; Immune system; Immuno-fluorescence; Ixodidae; Langerhans cells; Leukocyte; Leukocytes - cytology; Leukocytes - immunology; Lymphocytes; macrophages; Santa Gertrudis; Skin; Skin - cytology; Skin - immunology; skin lesions; Studies; T cell receptors; T-lymphocytes; T-Lymphocytes - cytology; T-Lymphocytes - immunology; taurine; tick infestations; Veterinary medicine; United States > US; California; Immuno-fluorescence; Skin; Breed; Leukocyte; Cattle
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2013.08.014

The immuno-staining patterns of skin leukocytes were investigated in three breeds of cattle: Holstein–Friesian, Brahman and Santa Gertrudis of similar age before and after tick infestation. The antibodies specific for CD45 and CD45RO reacted with cells in the skin of all Holstein–Friesian cattle but did not react with cells in the skin of any Brahman cattle. The same antibodies reacted with cells from the skin of four (CD45) and seven (CD45RO) of twelve Santa Gertrudis cattle. The antibodies specific for T cells and γδ subset of T cells recognized cells from all three breeds of cattle. The antibody specific for MHC class II molecules labelled cells of mostly irregular shape, presumably dermal dendritic cells and/or macrophages and Langerhans cells. The antibody specific for granulocytes (mAb CH138) reacted with cells only in sections cut from skin with lesions. The antibody specific for CD25+ cells labelled regularly shaped cells that showed a wide range of intensities of staining.