Comparison of the in vitro pathogenicity of two Salmonella Typhimurium phage types

• Published in: Comparative Immunology, Microbiology and Infectious Diseases Vol. 30; no. 1; pp. 11 - 18
• Main Authors: Vo, An T.T, van Duijkeren, Engeline, Fluit, Ad C, Hendriks, Henno G.C.J.M, Tooten, Peter C.J, Gaastra, Wim
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-01-2007; Elsevier
• Subjects: Adhesion; Adhérence; Allergy and Immunology; Animals; Bacterial Adhesion - physiology; Bacteriology; Biological and medical sciences; Caco-2 Cells; Cell Line; Cellule épithéliale; Epithelial cell; Fundamental and applied biological sciences. Psychology; Humans; IL-8; Infectious Disease; Interleukin-8 - biosynthesis; Invasion; Microbiology; Miscellaneous; Phage type; Salmonella enterica; Salmonella Phages - pathogenicity; Salmonella Typhimurium; Salmonella typhimurium - pathogenicity; Salmonella typhimurium - physiology; Species Specificity; Swine; Virology; Virulence; Phage type; Adhérence; Salmonella Typhimurium; Cellule épithéliale; Epithelial cell; IL-8; Adhesion; Phage-type; Invasion; Virus; Infection; Pathogenicity; Immunology; Microbiology; Bacteria; Salmonella typhimurium; Enterobacteriaceae; Phage
• ISSN: 0147-9571; 1878-1667; 1365-2567
• DOI: 10.1016/j.cimid.2006.09.003

Abstract The in vitro pathogenicity of Salmonella enterica serovar Typhimurium phage type (pt) 90 and pt 506 (also known as DT 104) isolates from human and porcine origin was studied in adhesion and invasion assays to the human cell line Caco-2 and the porcine cell line IPI-2. Interleukin-8 (IL-8) production by these two cell lines in response to stimulation by the two Salmonella phage types was also measured. Generally, Salmonella Typhimurium pt 506 and pt 90 adhered to and invaded Caco-2 cells and IPI-2 cells equally well. The release of IL-8 by Caco-2 cells or by IPI-2 cells was similar, independent of the Salmonella phage type used for stimulation of the cells. These data suggest that Salmonella Typhimurium pt 90 has a similar ability to cause Salmonella infections as Salmonella Typhimurium DT 104.