Proteome Differences of Placenta Between Pre-Eclampsia and Normal Pregnancy

• Published in: Placenta (Eastbourne) Vol. 31; no. 2; pp. 121 - 125
• Main Authors: Gharesi-Fard, B, Zolghadri, J, Kamali-Sarvestani, E
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-02-2010; Elsevier
• Subjects: 2D-PAGE; Adult; Biological and medical sciences; Cesarean Section; Down-Regulation; Electrophoresis, Gel, Two-Dimensional; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; HSC70 Heat-Shock Proteins - chemistry; HSC70 Heat-Shock Proteins - metabolism; Humans; Image Processing, Computer-Assisted; Internal Medicine; Mass spectrometry; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - metabolism; Obstetrics and Gynecology; Placenta; Placenta - metabolism; Pre-eclampsia; Pre-Eclampsia - metabolism; Pregnancy; Pregnancy Proteins - chemistry; Pregnancy Proteins - metabolism; Proteomics; Proteomics - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Term Birth; Up-Regulation; Young Adult; 2D-PAGE; Placenta; Pre-eclampsia; Mass spectrometry; Proteomics; Pregnancy; Vertebrata; Mammalia; Pregnancy disorders; Fetal membrane; Preeclampsia; Normal; Pregnancy toxemia
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/j.placenta.2009.11.004

Abstract Placenta is a tissue unique to pregnancy and despite its major role in pregnancy, little is known about the proteome changes within placenta during pregnancy-related diseases such as pre-eclampsia (PE). Therefore, the aim of this study is the analysis of proteome differences between pre-eclamptic and normal full-term placentas. To achieve this goal, five normal and five severe pre-eclamptic placentas were included in this study. Total placental proteins were extracted and subjected to two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). After staining, the gels were scanned and the protein spots were analysed using Image Master 2D Platinum Software. Non-parametric Mann–Whitney test was used for analysis of the mean intensity differences of the spots between normal and pre-eclamptic placentas. Statistical analysis indicated that 17 spots were differently expressed in pre-eclamptic compared with normal placentas ( p < 0.05). Using Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF/TOF) mass analysis, 11 out of 17 spots were identified. Among them, four proteins (chloride intracellular channel 3, apolipoprotein A-I, transthyretin (TTR) and protein disulphide isomerase) were up-regulated while seven (peroxiredoxin 2, peroxiredoxin 3, Hsc 70, Cu/Zn-superoxide dismutase (SOD-1), actin gamma 1 propeptide, chain A of enoyl-coenzyme A hydratase and HSP gp96) showed decreased expression in PE in comparison with normal placentas. In conclusion, down-regulation of proteins with anti-oxidant activities (peroxiredoxin 2 and peroxiredoxin 3) and altered expression of stress-response proteins (Hsc 70, Hsp gp96 and protein disulphide isomerase) might play an important role in the pathogenesis of PE.