Bioavailability and Pharmacokinetics of Dehydroepiandrosterone in the Cynomolgus Monkey

• Published in: The journal of clinical endocrinology and metabolism Vol. 88; no. 9; pp. 4293 - 4302
• Main Authors: Leblanc, Mathias, Labrie, Claude, Bélanger, Alain, Candas, Bernard, Labrie, Fernand
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01-09-2003; Copyright by The Endocrine Society
• Subjects: Administration, Oral; Adrenal Cortex Hormones - blood; Adrenal Cortex Hormones - metabolism; Androgens - blood; Animals; Biological and medical sciences; Biological Availability; Dehydroepiandrosterone - administration & dosage; Dehydroepiandrosterone - blood; Dehydroepiandrosterone - pharmacokinetics; Dehydroepiandrosterone Sulfate - blood; Dexamethasone - pharmacology; Estrogens - blood; Female; Hormones. Endocrine system; Injections, Intravenous; Macaca fascicularis; Medical sciences; Ovariectomy; Pharmacology. Drug treatments; Vertebrata; Dehydroepiandrosterone; Mammalia; Animal; Monkey; Primates; Bioavailability; Experimental study; Pharmacokinetics
• ISSN: 0021-972X; 1945-7197
• DOI: 10.1210/jc.2003-022012

We have studied the pharmacokinetics of dehydroepiandrosterone (DHEA) administered orally (PO), iv, and during a continuous iv infusion in ovariectomized cynomolgus monkeys under suppression of adrenal DHEA secretion with dexamethasone. The glucocorticoid induced a rapid suppression of serum cortisol, DHEA, and DHEA-sulfate (DHEA-S) as well as their metabolites, thus permitting to use this model to study the pharmacokinetic parameters of DHEA and its metabolites without significant interference by endogenous steroid levels.
After a single 10 mg iv dose of DHEA, the metabolic clearance rate and terminal half-life of DHEA were 99.9 ± 9.1 liter/d and 4.5 ± 0.3 h, respectively. Following a 50-mg DHEA PO dose, systemic availability was only 3.1 ± 0.4%. As shown by their high conversion ratios, the major circulating metabolites of DHEA are DHEA-S, androsterone glucuronide, and androstane-3α,17β-diol-glucuronide. The conversion ratios of androst-5-ene-3β,17β-diol, testosterone, dihydrotestosterone, and androstenedione are, in comparison, small. No transformation to estrogens could be detected in the circulation after either iv or PO DHEA administration.
The present data indicate that DHEA is transformed predominantly into androgens in peripheral tissues in ovariectomized cynomolgus monkeys with minimal (androgens) or no (estrogens) release of the bioactive steroids in the circulation. Furthermore, the present study supports the importance of measuring circulating androgen glucuronide derivatives to assess hormonal exposure of peripheral tissues to androgens after DHEA administration.