Mechanisms of human eosinophil migration induced by the combination of IL-5 and the endocannabinoid 2-arachidonoyl-glycerol

• Published in: Journal of allergy and clinical immunology Vol. 133; no. 5; pp. 1480 - 1482.e3
• Main Authors: Larose, Marie-Chantal, BSc, Turcotte, Caroline, BSc, Chouinard, François, PhD, Ferland, Claudine, BSc, Martin, Cyril, MSc, Provost, Véronique, PhD, Laviolette, Michel, MD, Flamand, Nicolas, PhD
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-05-2014; Elsevier; Elsevier Limited
• Subjects: Allergy and Immunology; Arachidonic Acids - immunology; Arachidonic Acids - pharmacology; Biological and medical sciences; Biosynthesis; Cell Movement - drug effects; Cell Movement - immunology; Chemokines; Cytokines; Endocannabinoids - pharmacology; Enzymes; Eosinophils - immunology; Experiments; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glycerides - pharmacology; Glycerol; Humans; Immunopathology; Interleukin-5 - pharmacology; Leukocytes; Lipids; Medical sciences; Metabolites; Migration; Phosphorylation; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Human; Immunopathology; Cytokine; Granulocyte; Glycerol; Cannabinoid; Eosinophil; Mechanism; Endogenous; Immunology; Interleukin 5; Endocannabinoid; Interleukin 2; Cell migration
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2013.12.1081

[...]we assessed whether 2-AG hydrolysis inhibition affected eosinophil migration. Forsell et al8 and Brunnstrom et al9 recently documented the production of novel 2-AG-derived 15-lipoxygenase metabolites. [...]the 15-lipoxygenase-derived lipidome of human eosinophils is likely more complex than initially thought.\n HBSS, FBS, and RPMI 1640 were obtained from Wisent Laboratories (St-Bruno, Quebec, Canada).