Acetic acid, vinegar, and citric acid as washing materials for cuticle removal to improve hatching performance of quail eggs

• Published in: Poultry science Vol. 99; no. 8; pp. 3865 - 3876
• Main Authors: He, Zhaoxiang, Chen, Xia, Shi, Xuefeng, Li, Xianyu, Li, Chengfeng, Li, Junying, Xu, Guiyun, Yang, Ning, Zheng, Jiangxia
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01-08-2020; Elsevier
• Subjects: Acetic Acid - pharmacology; Animal Husbandry - methods; Animals; chick quality; Citric Acid - pharmacology; Egg Shell - drug effects; Egg Shell - microbiology; egg washing; eggshell cuticle; Female; hatchability; Management and Production; Microbiota - drug effects; Quail; chick quality; eggshell cuticle; egg washing; hatchability; quail
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2020.04.018

The cuticle is the outmost layer of the eggshell and may affect the hatchability by modulating eggshell conductance. Three different solutions using acetic acid (AA), vinegar (V), and citric acid (CA) for cuticle removal by egg washing were developed, and the effects of cuticle removal on hatching performance of quail hatching eggs were evaluated. A total of 5,238 fresh quail hatching eggs were randomly divided into 9 treatments as follows: unwashed control, nondipped (CND); washed control, water dipped (CWD); standard control, 0.13% sodium hyperchlorite (CSH); 2% AA (AA2); 4% AA (AA4); 44.4% V (V2); 88.8% V (V4); 2% CA (CA2); and 4% CA (CA4). Overall, AA4, V4, and CA4 treatments significantly improved the hatchability of fertile eggs (95.42%, 94.16% and 95.66%, respectively) (P < 0.05) and the hatchability of CND, CWD, CSH, AA2, V2 and CA2 treatments were 90.98%, 93.00%, 92.27%, 79.44%, 90.37%, and 90.59%, respectively. The eggshell thickness and cuticle quality results showed that all AA, V, and CA solutions can effectively remove the quail eggshell cuticle, and AA4, V4 and CA4 significantly decreased eggshell thickness (P < 0.05). Microbial activity on the eggshell surface in all acid treatments was reduced significantly at day 0 of incubation (P < 0.05) and that significantly decreased than controlled treatments over the incubation period except AA2 treatment.
Egg weight loss was lower for all acid treatments than that of the CND treatment (P < 0.05). There was no clear effect of treatments on chick quality. Hatch time in AA4, V4, and CA4 treatments slightly improved compared with controlled treatments (P > 0.05). There were no significant differences between treatments for chick livability and live weight at the first 21 D of life. Results of the present study indicate that cuticle removal with AA4, V4, or CA4 could effectively decrease the microbial activity on the eggshell surface during the incubation period and improve hatchability of quail hatching eggs without negative effects on hatch time and performance of quail chicks.