Multi-Omics Approaches for Revealing the Epigenetic Regulation of Histone H3.1 during Spermatogonial Stem Cell Differentiation In Vitro

• Published in: International journal of molecular sciences Vol. 24; no. 4; p. 3314
• Main Authors: Liu, Li, Li, Haojie, Wang, Mengjie, Zhang, Xiangzheng, Ren, Jie, Yuan, Yan, Sha, Jiahao, Guo, Xuejiang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07-02-2023; MDPI
• Subjects: Adult Germline Stem Cells; Binding; Cell differentiation; Cell growth; Chromosomes; differentiation; Differentiation (biology); DNA binding proteins; DNA methylation; Epigenesis, Genetic; Epigenetic inheritance; epigenetic regulation; Epigenetics; Gene expression; Histone H3; histone modification; Histones; Histones - metabolism; Kinases; Mass Spectrometry; Mass spectroscopy; Multiomics; Peptides; Pharmaceutical industry; Post-translation; Protein binding; Protein Processing, Post-Translational; Proteins; Proteomics; RNA; RNA polymerase; Scientific equipment and supplies industry; Spermatogenesis; Spermatogonia; spermatogonial stem cell; Stem cells; transcription factor; Transcription factors; Minnesota; United States; Massachusetts; China; Germany; transcription factor; differentiation; spermatogonial stem cell; histone modification; epigenetic regulation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24043314

Epigenetic regulation, particularly post-translational modifications (PTMs) of histones, participates in spermatogonial stem cell (SSCs) differentiation. However, there is a lack of systemic studies of histone PTM regulation during the differentiation of SSCs due to its low number in vivo. Herein, we quantified dynamic changes of 46 different PTMs on histone H3.1 by targeted quantitative proteomics using mass spectrometry during SSCs differentiation in vitro, in combination with our RNA-seq data. We identified seven histone H3.1 modifications to be differentially regulated. In addition, we selected H3K9me2 and H3S10ph for subsequent biotinylated peptide pull-down experiments and identified 38 H3K9me2-binding proteins and 42 H3S10ph-binding proteins, which contain several transcription factors, such as GTF2E2 and SUPT5H, which appear to be crucial for epigenetic regulation of SSC differentiation.