Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties

• Published in: npj Regenerative medicine Vol. 6; no. 1; p. 42
• Main Authors: Brown, Wendy E., Huang, Brian J., Hu, Jerry C., Athanasiou, Kyriacos A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06-08-2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/61/2035; 631/61/490; 631/61/54/994; Biomaterials; Biomedical and Life Sciences; Biomedicine; Cartilage; Cell Biology; Immunology; Knee; Regenerative Medicine/Tissue Engineering; Shear strength; Stem Cells
• ISSN: 2057-3995; 2057-3995
• DOI: 10.1038/s41536-021-00152-0

Despite the prevalence of large (>5 cm
2
) articular cartilage defects involving underlying bone, current tissue-engineered therapies only address small defects. Tissue-engineered, anatomically shaped, native-like implants may address the need for off-the-shelf, tissue-repairing therapies for large cartilage lesions. This study fabricated an osteochondral construct of translationally relevant geometry with robust functional properties. Scaffold-free, self-assembled neocartilage served as the chondral phase, and porous hydroxyapatite served as the osseous phase of the osteochondral constructs. Constructs in the shape and size of an ovine femoral condyle (31 × 14 mm) were assembled at day 4 (early) or day 10 (late) of neocartilage maturation. Early osteochondral assembly increased the interfacial interdigitation depth by 244%, interdigitation frequency by 438%, interfacial shear modulus by 243-fold, and ultimate interfacial shear strength by 4.9-fold, compared to late assembly. Toward the development of a bioprosthesis for the repair of cartilage lesions encompassing up to an entire condylar surface, this study generated a large, anatomically shaped osteochondral construct with robust interfacial mechanical properties and native-like neocartilage interdigitation.