Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

• Published in: Data in brief Vol. 7; pp. 60 - 65
• Main Authors: Church, Molly E., Dela Cruz, Florante N., Kim, Kevin, Persiani, Michele, Woods, Leslie W., Pesavento, Patricia A., Woolard, Kevin D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-06-2016; Elsevier
• Subjects: Data
• ISSN: 2352-3409; 2352-3409
• DOI: 10.1016/j.dib.2016.01.070

Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.