A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs

• Published in: Nature (London) Vol. 334; no. 6179; pp. 218 - 224
• Main Authors: Santoro, Claudio, Mermod, Nicolas, Andrews, Philip C, Tjian, Robert
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 21-07-1988; Nature Publishing Group
• Subjects: Adenoviridae - genetics; Amino Acid Sequence; Base Sequence; Biochemistry; Biological and medical sciences; Cloning; Cloning, Molecular; Deoxyribonucleic acid; DNA; DNA - genetics; DNA Replication; DNA-Binding Proteins - genetics; Fundamental and applied biological sciences. Psychology; Genetics; Humans; Medical research; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Promoter Regions, Genetic; Proteins; RNA Polymerase II - metabolism; RNA Splicing; Transcription Factors - genetics; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Virus Replication; Human; Regulation(control); Multiple; Messenger RNA; Complementary DNA; Splicing; Gene product; Replication initiation; DNA binding protein; RNA polymerase 2; Trans acting regulatory factor; Transcription initiation
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/334218a0

The CTF/NF-I group of cellular DNA binding proteins recognizes the sequence GCCAAT and is implicated in eukaryotic transcription as well as DNA replication. Molecular analysis of human CTF/NF-I complementary DNA clones reveals multiple messenger RNA species containing alternative coding regions, apparently as a result of differential splicing. Expression and functional analysis establish that individual gene products can bind to GCCAAT recognition sites and serve both as promoter-selective transcriptional activators and as initiation factors for DNA replication.