Erythropoietin Attenuates Apoptosis After Ischemia-Reperfusion–Induced Renal Injury in Transiently Hyperglycemic Wister Rats

• Published in: Transplantation proceedings Vol. 43; no. 10; pp. 3618 - 3621
• Main Authors: Caetano, A.M.M, Vianna Filho, P.T.G, Castiglia, Y.M.M, Golim, M.A, de Souza, A.V.G, Raquel de Carvalho, L, Deffune, E, de Oliveira, C, Vianna, P.T.G
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01-12-2011; Elsevier
• Subjects: Animals; Apoptosis - drug effects; Biological and medical sciences; Blood Glucose - metabolism; Creatinine - blood; Cytoprotection; Disease Models, Animal; Epoetin Alfa; Erythropoietin - pharmacology; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Hyperglycemia - complications; Kidney - blood supply; Kidney - drug effects; Kidney - pathology; Kidney - surgery; Kidney Glomerulus - drug effects; Kidney Glomerulus - pathology; Kidney Tubules - drug effects; Kidney Tubules - pathology; Male; Medical sciences; Necrosis; Nephrectomy - adverse effects; Rats; Rats, Wistar; Recombinant Proteins - pharmacology; Reperfusion Injury - etiology; Reperfusion Injury - pathology; Reperfusion Injury - prevention & control; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Time Factors; Tissue, organ and graft immunology; Temperature; Erythropoietin; Cold; Rat; Hyperglycemic substances; Rodentia; Environmental factor; Cardiovascular disease; Transplantation; Kidney; Medicine; Vertebrata; Hyperglycemia; Mammalia; Reperfusion; Treatment; Urinary system; Ischemia; Polypeptide; Cell death; Surgery; Animal; Graft; Apoptosis
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2011.10.049

Abstract Background Hyperglycemia is associated with a decreased tolerance to ischemia and an increased severity of renal ischemia reperfusion (I/R) injury. It has been suggested that erythropoietin (EPO) attenuates this effect in normoglycemic animals. This study sought to examine the effects of EPO on treatment renal I/R injury (IRI) in transiently hyperglycemic rats. Material and Methods Twenty-eight male Wister rats anesthetized with isoflurane received glucose (2.5 g.kg−1 intraperitoneally) before right nephrectomy. They were randomly assigned to four groups: sham operation (S); IRI (ISO); IRI+EPO, (600 UI kg−1 low-dose EPO [EL]); and IRI+EPO 5000 UI kg−1 (high-dose EPO [EH]). IRI was induced by a 25-minute period of left renal ischemia followed by reperfusion for 24 hours. Serum Creatinine and glucose levels were measure at baseline (M1), immediately after the ischemic period (M2), and at 24 hours after reperfusion (M3). After sacrificing the animals, left kidney specimens were submitted for histological analysis including flow cytometry to estimate tubular necrosis and the percentages of apoptotic, dead or intact cells. Results Scr in the ISO group was significantly higher at M3 than among the other groups. Percentages of early apoptotic cells in ISO group were significantly higher than the other groups. Percentages of late apoptotic cells in S and ISO groups were significantly greater than EL and EH groups. However, no significant intergroup differences were observed regarding the incidence of tubular necrosis. Conclusions Our results suggested that, although not preventing the occurrence of tubular necrosis, EPO attenuated apoptosis and glomerular functional impairment among transiently hyperglycemic rats undergoing an ischemia/reperfusion insult.