Quantification of Adenovirus DNA in Plasma for Management of Infection in Stem Cell Graft Recipients

• Published in: Clinical infectious diseases Vol. 34; no. 6; pp. 864 - 867
• Main Authors: Lankester, A. C., van Tol, M. J. D., Claas, E. C. J., Vossen, J. M., Kroes, A. C. M.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 15-03-2002; University of Chicago Press
• Subjects: Adenoviridae - genetics; Adenoviridae - physiology; Adenovirus Infections, Human - drug therapy; Adenoviruses; Antivirals; Biological and medical sciences; Blood plasma; Bone marrow; Brief Reports; Child; DNA, Viral - blood; Drug Monitoring; Hematopoietic Stem Cell Transplantation; Human viral diseases; Humans; Infections; Infectious diseases; Male; Medical sciences; Miscellaneous; Pediatrics; Polymerase Chain Reaction; Quantification; Symptoms; Transplants; Viral diseases; Viral Load; Viruses; Adenoviridae; Stem cell; Homograft; Recipient; Blood plasma; Infection; Virus; Clinical management; DNA; Viral disease; Diagnosis; Viral load; Quantitative analysis
• ISSN: 1058-4838; 1537-6591
• DOI: 10.1086/339073

We used a real-time polymerase chain reaction method for quantification of adenovirus to monitor the dynamics of viral DNA load in plasma in pediatric stem-cell graft recipients. Two cases are described to demonstrate that detection and quantification of the adenovirus DNA load at regular intervals may be important to document the stage of adenovirus infection, to make decisions on clinical intervention, and to accurately monitor the response to antiviral therapy.