Activation of the 2-5OAS/RNase L pathway in CVB1 or HAV/18f infected FRhK-4 cells does not require induction of OAS1 or OAS2 expression

• Published in: Virology (New York, N.Y.) Vol. 388; no. 1; pp. 169 - 184
• Main Authors: Kulka, Michael, Calvo, Mona S, Ngo, Diana T, Wales, Samantha Q, Goswami, Biswendu B
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25-05-2009
• Subjects: 2',5'-Oligoadenylate Synthetase - metabolism; 2-5A; 60 APPLIED LIFE SCIENCES; Animals; Antiviral; Cell Line; Coxsackievirus; Cytopathogenic Effect, Viral; DIGESTIVE SYSTEM DISEASES; DISEASES; Endoribonucleases - metabolism; Enterovirus B, Human - physiology; Enzyme Activation - physiology; Enzyme Induction - physiology; ENZYMES; ESTERASES; HEPATITIS; Hepatitis A virus; Hepatitis A virus - physiology; HYDROLASES; Infectious Disease; Interferons; Kidney - cytology; LIGASES; Macaca mulatta; MICROORGANISMS; NUCLEASES; Oligoadenylate synthetase; ORGANIC COMPOUNDS; PARASITES; PHOSPHODIESTERASES; PROTEINS; RNA, Double-Stranded; RNA-ASE; RNase L; VIRUSES; 2-5A; Coxsackievirus; Antiviral; Oligoadenylate synthetase; Hepatitis A virus; RNase L
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2009.03.014

Abstract The latent, constitutively expressed protein RNase L is activated in coxsackievirus and HAV strain 18f infected FRhK-4 cells. Endogenous oligoadenylate synthetase (OAS) from uninfected and virus infected cell extracts synthesizes active forms of the triphosphorylated 2-5A oligomer (the only known activator of RNase L) in vitro and endogenous 2-5A is detected in infected cell extracts. However, only the largest OAS isoform, OAS3, is readily detected throughout the time course of infection. While IFNβ treatment results in an increase in the level of all three OAS isoforms in FRhK-4 cells, IFNβ pretreatment does not affect the temporal onset or enhancement of RNase L activity nor inhibit virus replication. Our results indicate that CVB1 and HAV/18f activate the 2-5OAS/RNase L pathway in FRhK-4 cells during permissive infection through endogenous levels of OAS, but contrary to that reported for some picornaviruses, CVB1 and HAV/18f replication is insensitive to this activated antiviral pathway.