Comparison of Two Cutibacterium acnes Biofilm Models

• Published in: Microorganisms (Basel) Vol. 9; no. 10; p. 2035
• Main Authors: Varin-Simon, Jennifer, Lamret, Fabien, Colin, Marius, Gangloff, Sophie C., Mongaret, Céline, Reffuveille, Fany
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 26-09-2021; MDPI
• Subjects: Bacteria; biofilm models; Biofilms; Biomedical materials; Bone surgery; Communication; Cutibacterium acnes; Dynamic models; host-pathogen interactions; Infections; Internalization; Life Sciences; Microscopy; Prostheses; Static models; Virulence; United States > US; Biofilm models; host-pathogen interactions; Cutibacterium acnes
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms9102035

The study of biofilms in vitro is complex and often limited by technical problems due to simplified models. Here, we compared C. acnes biofilm formation, from species involved in bone and prosthesis infection, in a static model with a dynamic model. Using similar parameters, the percentage of live bacteria within the biofilm was higher in dynamic than in static approach. In both models, bacterial internalization in osteoblast-like cells, playing the role of stress factor, affected this proportion but in opposite ways: increase of live bacteria proportion in the static model (×2.04 ± 0.53) and of dead bacteria proportion (×3.5 ± 1.03) in the dynamic model. This work highlights the huge importance in the selection of a relevant biofilm model in accordance with the environmental or clinical context to effectively improve the understanding of biofilms and the development of better antibiofilm strategies.